In vitro flowering of Physalis angulata L. (Solanaceae)
DOI:
https://doi.org/10.70151/r64g9g47Keywords:
Axillary bud, juvenility, medicinal plant, Solanaceae, tissue cultureAbstract
Germination of Physalis angulata in vitro in Murashige & Skoog medium (MS) showed positive photoblastic characteristics. These plants were cultured using the single-node method in MS medium. The interval between subcultures was 20 days and the average multiplication rate obtained through 4 subcultures was 1: 3.8 plantlets. Flowering of Physalis angulata occurred 3 months after the 4th subculture, showing 0.6 mm floral buds. The mature state of the explant source was found to be fundamental for in vitro flowering. After 5th subculture, the rate of flowering from apexes was 50% and from nodal segments, 45%. The development of floral buds was higher and faster from nodal segments than apexes, the first anthesis occurring after 21 days. The in vitro flowers showed normal development and viable pollen. Plantlets transferred to soil accomplished the vegetative cycle, producing fruits after 3 months. Growing regulator was not added along the experimental procedure.
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