Doses and sources of organic fertilization influence biomass, volatile chemical composition, and phenolic compounds in Lippia gracilis

Authors

DOI:

https://doi.org/10.70151/bpx45w25

Keywords:

‘Alecrim da chapada’, Organic fertilization, Growth indices, Chemical analysis, Essential oil

Abstract

Lippia gracilis has emerged as one of the most important medicinal plants of the genus. Organic farming systems are currently growing substantially, aiming to obtain sustainable and economically viable plant production. Thus, the objective of this study was to assess the effect of different doses and sources of poultry manure on biomass production, photosynthetic pigment levels, phenolic content and volatile chemical composition in L. gracilis. The experiment was conducted in a completely randomized design in a 2 × 4 factorial arrangement with two sources of poultry manure (quail and chicken) and four doses (0.0, 1.5, 3.0, and 6.0 kg/m2) in 10 l pots. Plants fertilized with quail manure at a dose of 1.5 – 3.0 kg/m2 accumulated more total dry weight, while for chicken manure, the highest total dry weight gains were observed at doses of 3.0 and 6.0 kg/m2. In the chemical analyses of the essential oil, the major chemical constituent identified in all treatments was carvacrol. However, the highest carvacrol content was observed in the control treatment. L. gracilis plants increase their biomass when grown with 1.5 kg/m2 of quail manure or 3.0 to 6.0 kg/m2 of chicken manure. However, fertilization reduces carvacrol levels.

Downloads

Download data is not yet available.

Author Biographies

  • Brígida Resende Almeida, Federal University of Lavras

    Laboratory of Phytochemistry and Medicinal Plants, Department of Agriculture

  • Rafael Marlon Alves de Assis, Federal University of Lavras

    Laboratory of Plant Tissue Culture and Medicinal Plants, Department of Agriculture

  • Jeremias José Ferreira Leite, Federal University of Lavras

    Laboratory of Plant Tissue Culture and Medicinal Plants, Department of Agriculture

  • Alexandre Alves de Carvalho, Federal University of Lavras

    Laboratory of Plant Tissue Culture and Medicinal Plants, Department of Agriculture

  • Suzan Kelly Vilela Bertolucci, Federal University of Lavras

    Laboratory of Phytochemistry and Medicinal Plants, Department of Agriculture

  • Jose Eduardo Brasil Pereira Pinto, Federal University of Lavras

    Laboratory of Plant Tissue Culture and Medicinal Plants, Department of Agriculture

References

Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectrometry. Allured publishing corporation Carol Stream, IL; Illinois

Ahn M-R, Kumazawa S, Usui Y, Nakamura J, Matsuka M, Zhu F, Nakayama T (2007) Antioxidant activity and constituents of propolis collected in various areas of China. Food Chem 101:1383-1392. https://doi.org/10.1016/j.foodchem.2006.03.045

Albuquerque CCd, Camara TR, Mariano RdLR, Willadino L, Marcelino Júnior C, Ulisses C (2006) Antimicrobial action of the essential oil of Lippia gracilis Schauer. Braz Arch Biol Technol 49:527-535. https://doi.org/10.1590/S1516-89132006000500001

Bibiano CS, de Carvalho AA, Bertolucci SKV, Torres SS, Corrêa RM, Pinto JEBP (2019) Organic manure sources play fundamental roles in growth and quali-quantitative production of essential oil from Dysphania ambrosioides L. Ind Crops Prod 139:111512. https://doi.org/10.1016/j.indcrop.2019.111512

Bitu VdC, Fecundo HD, da Costa JG, Coutinho HD, Rodrigues FF, de Santana NM, Botelho MA, Menezes IR (2014) Chemical composition of the essential oil of Lippia gracilis Schauer leaves and its potential as modulator of bacterial resistance. Nat Prod Res 28:399-402. https://doi.org/10.1080/14786419.2013.867343

Bitu VdCN, Martins da Costa JG, Rogrigues FFG, Colares AV, Coutinho HDM, Botelho MA, Portela AdC, de Santana NM, Menezes IRA (2015) Effect of collection time on composition of essential oil of Lippia gracilis Schauer (Verbenaceae) growing in northeast Brazil. J Essent Oil-Bear Plants 18:647-653. https://doi.org/10.1080/0972060X.2014.935043

Brant RdS, Pinto JEB, Bertolucci SK, Albuquerque CJB (2010) Biomass production and essential oil content of lemon verbena in response to organic manure application. Hortic Bras 28:111-114. https://doi.org/10.1590/S0102-05362010000100021

Chagas JH, Pinto JEB, Bertolucci SK, Santos FM, Botrel PP, Pinto LB (2011) Production of mint depending on organic manure at planting and dressing. Hortic Bras 29:412-417. https://doi.org/10.1590/S0102-05362011000300026

Corrêa, RM, Pinto, JEBP, Reis, ÉS, Oliveira, CD, Castro, EMD, Brant, RDS (2009) Características anatômicas foliares de plantas de orégano (Origanum vulgare L.) submetidas a diferentes fontes e níveis de adubação orgânica. Acta Scientiarum. Agronomy 31:439-444. doi:10.4025/actasciagron.v31i3.690

Costa AG, Bertolucci SKV, Chagas JH, Ferraz EO, Pinto JEBP (2013) Biomass production, yield and chemical composition of peppermint essential oil using different organic fertilizer sources. Cienc Agrotec 37:202-210. https://doi.org/10.1590/S1413-70542013000300002

Costa LCdB, Pinto JEBP, Castro EMd, Bertolucci SKV, Corrêa RM, Reis ÉS, Alves PB, Niculau EdS (2008) Sources and doses of organic fertilization in Ocimum selloi growth, essential oil yield and chemical composition. Cienc Rural 38:2173-2180. https://doi.org/10.1590/S0103-84782008000800013

Cruz EM, Costa LM, Jr., Pinto JA, Santos Dde A, de Araujo SA, Arrigoni-Blank Mde F, Bacci L, Alves PB, Cavalcanti SC, Blank AF (2013) Acaricidal activity of Lippia gracilis essential oil and its major constituents on the tick Rhipicephalus (Boophilus) microplus. Vet Parasitol 195:198-202. https://doi.org/10.1016/j.vetpar.2012.12.046

Ferreira DF (2011) Sisvar: a computer statistical analysis system. Cienc Agrotec 35:1039-1042. https://doi.org/10.1590/S1413-70542011000600001

Guimarães AG, Gomes SVF, Moraes VRS, Nogueira PCL, Ferreira AG, Blank AF, Santos ADC, Viana MD, Silva GH, Quintans Júnior LJ (2012) Phytochemical characterization and antinociceptive effect of Lippia gracilis Schauer. J Nat Med 66:428-434. https://doi.org/10.1007/s11418-011-0601-3

Hawkesford M, Horst W, Kichey T, Lambers H, Schjoerring J, Møller IS, White P (2012) Chapter 6 - Functions of Macronutrients. In Marschner P (ed) Marschner's mineral nutrition of higher plants Academic Press, San Diego, p.135-189

Hokmalipour, S; Darbandi, MH. (2011) Effects of nitrogen fertilizer on chlorophyll content and other leaf indicate in three cultivars of maize (Zea mays L.). World Applied Sci J 12: 1780-1785.

Kostić DA, Dimitrijević DS, Mitić SS, Mitić MN, Stojanović GN, Živanović AV (2013) A survey on macro- and micro-elements, phenolic compounds, biological activity and use of Morus spp. (Moraceae). Fruits 68:333-347. https://doi.org/10.1051/fruits/2013079

Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. Cur Prot Food Anal Chem 1:F4.3.1-F4.3.8. https://doi.org/10.1002/0471142913.faf0403s01

Lopes V, Bertolucci SKV, de Carvalho AA, Roza HLH, Figueiredo FC, Pinto J (2019) Improvement of Cymbopogon flexuosus biomass and essential oil production with organic manures. J Agric Sci 11https://doi.org/10.5539/jas.v11n2p169

Maia SS, Pinto JE, da Silva FN, de Oliveira C (2008) Influence of the organic and mineralfertilization on the growth of Hyptissuaveolens (L.) Poit. (Lamiaceae). Rev Bras Cienc Agrar 3:327-331.

Melo JO, Fachin AL, Rizo WF, Jesus HC, Arrigoni-Blank MF, Alves PB, Marins MA, França SC, Blank AF (2014) Cytotoxic effects of essential oils from three Lippia gracilis Schauer genotypes on HeLa, B16, and MCF-7 cells and normal human fibroblasts. Genet Mol Res 13:2691-2697. https://doi.org/10.4238/2014.April.8.12

Mendes SS, Bomfim RR, Jesus HC, Alves PB, Blank AF, Estevam CS, Antoniolli AR, Thomazzi SM (2010) Evaluation of the analgesic and anti-inflammatory effects of the essential oil of Lippia gracilis leaves. J Ethnopharmacol 129:391-397. https://doi.org/10.1016/j.jep.2010.04.005

Miguel M, Bouchmaaa N, Aazza S, Gaamoussi F, Lyoussi B (2014) Antioxidant, anti-inflammatory and anti-acetylcholinesterase activities of eleven extracts of Moroccan plants. Fresenius Environ Bull 23:1-14.

NIST (2008) PC version 2.0 of the NIST/EPA/NIH mass spectral library. National Institute of Standards and Technology Gaithersburg,

Pandey V, Patel A, Patra DD (2016) Integrated nutrient regimes ameliorate crop productivity, nutritive value, antioxidant activity and volatiles in basil (Ocimum basilicum L.). Ind Crops Prod 87:124-131. https://doi.org/10.1016/j.indcrop.2016.04.035

Pascual ME, Slowing K, Carretero E, Sánchez Mata D, Villar A (2001) Lippia: traditional uses, chemistry and pharmacology: a review. J Ethnopharmacol 76:201-214. https://doi.org/10.1016/S0378-8741(01)00234-3

Pessuto, MB, Costa, ICD, Souza, ABD, Nicoli, FM, Petereit, F, Luftmann H, Mello, JCP (2009) Atividade Antioxidante de extratos e taninos condensados das folhas de Maytenus ilicifolia Mart. ex reiss. Quim Nova 32:412-416.

Popova M, Bankova V, Butovska D, Petkov V, Nikolova-Damyanova B, Sabatini AG, Marcazzan GL, Bogdanov S (2004) Validated methods for the quantification of biologically active constituents of poplar-type propolis. Phytochem Anal 15:235-240. https://doi.org/10.1002/pca.777

Prista LVN, Alves AC, Lobo JS, Morgado R (2011) Pharmaceutical technology. 7 ed. Fundação Calouste Gulbenkian; Lisboa, Portugal.

Ragagnin RCG, Albuquerque CC, Oliveira FFM, Santos RG, Gurgel EP, Diniz JC, Rocha SAS, Viana FA (2014) Effect of salt stress on the growth of Lippia gracilis Schauer and on the quality of its essential oil. Acta Bot Brasilica 28:346-351. https://doi.org/10.1590/0102-33062014abb3369

Roby MHH, Sarhan MA, Selim KA-H, Khalel KI (2013) Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts. Ind Crops Prod 43:827-831. https://doi.org/10.1016/j.indcrop.2012.08.029

Rosal LF, Pinto JEBP, Bertolucci SKV, Brant RdS, Niculau EdS, Alves PB (2011) Effect of organic fertilizer sources on biomass and essential oil production of Plectranthus neochilus Schlechter. Rev Ceres 58:670-678. https://doi.org/10.1590/S0034-737X2011000500020

Santos CPd, Pinto JAO, Santos CAd, Cruz EMO, Arrigoni-Blank MdF, Andrade TM, Santos DdA, Alves PB, Blank AF (2016) Harvest time and geographical origin affect the essential oil of Lippia gracilis Schauer. Ind Crops Prod 79:205-210. https://doi.org/10.1016/j.indcrop.2015.11.015

Santos MM, Peixoto AR, Pessoa EdS, Nepa HBdS, Paz CDd, Souza AVVd (2014) Studies of chemical compounds and antibacterial activity of the essential oil from Lippia gracilis against Xanthomonas campestris pv. viticola "in vitro". Summa Phytopathol 40:277-280. https://doi.org/10.1590/0100-5405/1958

Silva WJ, Dória GAA, Maia RT, Nunes RS, Carvalho GA, Blank AF, Alves PB, Marçal RM, Cavalcanti SCH (2008) Effects of essential oils on Aedes aegypti larvae: Alternatives to environmentally safe insecticides. Bioresour Technol 99:3251-3255. https://doi.org/10.1016/j.biortech.2007.05.064

Singh K, Chand S, Yaseen M (2014) Integrated nutrient management in Indian basil (Ocimum basilicum). Ind Crops Prod 55:225-229. https://doi.org/10.1016/j.indcrop.2014.02.009

Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 16:144.

Sucupira, NR, Silva, AB, Pereira, G, Costa, JN. (2012) Métodos para determinação da atividade antioxidante de frutos. UNOPAR Científica. Ciências biológicas e da saúde,14:263-269.

Sujatha S, Bhat R, Kannan C, Balasimha D (2011) Impact of intercropping of medicinal and aromatic plants with organic farming approach on resource use efficiency in arecanut (Areca catechu L.) plantation in India. Ind Crops Prod 33:78-83. https://doi.org/10.1016/j.indcrop.2010.09.001

Teles S, Pereira JA, Muniz de Oliveira L, Malheiro R, Lucchese AM, Silva F (2014) Lippia origanoides H.B.K. essential oil production, composition, and antioxidant activity under organic and mineral fertilization: Effect of harvest moment. Ind Crops Prod 60:217-225. https://doi.org/10.1016/j.indcrop.2014.06.008

Van Den Dool H, Dec. Kratz P (1963) A generalization of the retention index system including linear temperature programmed gas—liquid partition chromatography. J Chromatogr A 11:463-471. https://doi.org/10.1016/S0021-9673(01)80947-X

Downloads

Published

2024-09-17

Issue

Vol. 24 No. 4 (2022)

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Doses and sources of organic fertilization influence biomass, volatile chemical composition, and phenolic compounds in Lippia gracilis. (2024). Revista Brasileira De Plantas Medicinais, 24(4), 182-193. https://doi.org/10.70151/bpx45w25