Chemical constituents and cytotoxic potential of essential oil Xylopia laevigata in hepatocellular carcinoma cells - HepG2
DOI:
https://doi.org/10.70151/d1452f06Keywords:
cytotoxic potential, Natural Products, Essential Oils, Antitumor ActivityAbstract
The Xylopia laevigata (Mart.) R. E. Frie, known as pindaíba or meiu is a native plant of the Atlantic Forest and popularly applied as a medicinal plant in some regions of the country. The essential oil extracted from the leaves of this plant was characterized in order to determine their chemical composition and evaluated the cytotoxic potential of the essential oil, the front line HepG2 hepatocellular carcinoma (liver cancer). The essential oil was extracted by hydrodistillation and components identified by gas chromatography with FID detector and gas chromatography coupled to mass spectrometry. The major identified compounds were bicyclogermacrene, β-caryophyllene, Germacrene-D, spatulenol and α-amorfene. To assess cell viability was used XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide), which checks the cellular redox potential. All concentrations 62.5 mg/ml to 250 mg/ml, the inhibition occurred in 100 % cells of hepatocellular carcinoma. These results direct the subsequent phytochemical studies to confirm their cytotoxic potential and their possible applications in antitumor activity.
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