Comparison of the cytotoxicity of the medicinal plant Poincianella pyramidalis and the endophytic fungus Penicillium sp. in macrophages of the J774 lineage
DOI:
https://doi.org/10.70151/vtfcmc97Keywords:
Poincianella pyramidalis, Penicillium, cytotoxicityAbstract
The aim of the present study was to evaluate the cytotoxic activity in vitro of the ethanolic extract of the plant Poincianella pyramidalis and of ethyl acetate extracts from the fermentation broth of endophytic fungi Penicillium sp. isolated from P. pyramidalis. This was a basic in vitro experimental research with a quantitative approach. The research consisted of macroscopic analysis assays associated with microscopic analysis to identify the taxonomy of isolated fungi as well as cell viability assay to evaluate the cytotoxic activity by the colorimetric method Methyltetrazolium (MTT) with macrophages of the J774 lineage. In the cytotoxic assay, the two extracts tested with the J774 macrophages strain proved to be non-toxic in all concentrations tested (100, 10 e 1 μg/mL). It is concluded that the medicinal plant P. pyramidalis harbor endophytic fungi Penicillium sp. and the fungal extract and ethanolic extract of the plant that were tested did not present deleterious effects to J774 macrophages.
Downloads
References
Alvin A, Miller KI, Neilan BA (2014) Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds. Microbiol Res 169(7-8):483-495. https://doi.org/10.1016/j.micres.2013.12.009
Aversi-Ferreira TA, Ribeiro PP, Silva NC, Brandão LD, Gratão LH, Nyamdavaa E, Aversi-Ferreira RA, Nishio H, Nascimento GNL (2013). Confrontation between ethnopharmacology and scientific results of the herbal medicaments from Brazil to be applied in primary health care. J Med Plants Res 7(14):845-856. https://doi.org/10.5897/JMPR12.392
De Hoog GS, Guarro J, Gené J, Ahmed SA, Al-Hatmi AMS, Figueras MJ, Vitale RG (2000) Atlas of clinical fungi. Utrech: Centraalbureau voor schimmelcultures. 1126p.
De Stefano S, Nicoletti R, Milone A, Zambardino, S (1999) 3-O-Methylfunicone, a fungitoxic metabolite produced by the fungus Penicillium pinophilum. Phytochemistry 52:1399-1401. https://doi.org/10.1016/S0031-9422(99)00320-9
Dobranic JK, Johnson JA, Alikhan QR. (1995). Isolation of endophytic fungi from eastern larch (Larix laricina) leaves fom New Brunswick, Canada. Can J Microbiol 41:194-198. https://doi.org/10.1139/m95-026
Domsch KH, Gams W, Traute-Heidi A (1980) Compendium of Soil Fungi. New York: APS Press. 859p.
Gallo MBC, Chagas FO, Almeida MO et al. (2009). Endophytic fungi found in association with Smallanthus sonchifolius (Asteraceae) as resourceful producers of cytotoxic bioactive natural products. J Basic Microb 49:142-151. https://doi.org/10.1002/jobm.200800093
Guerin P (1898) Sur la presence d’un champignon dans l’ivraie. Journal Botanique 12:230–238.
Guijarro B, Melgarejo R, Torres P, Lamarca N, Usall J, De Cal A (2008) Penicillium frequentans population dynamics on peach fruits after its applications against brown rot in orchards. J Appl Microbiol 104:659–671. https://doi.org/10.1111/j.1365-2672.2007.03596.x
Gunatilaka AAL (2006) Natural products from plant-associated microorganisms: distribution, structural diversity, bioactivity, and implications of their occurrence. J Nat Prod 69:509-526. https://doi.org/10.1021/np058128n
Huang WY, Cai YZ, Hyde KD, Cork H, Sun M (2007) Endophytic fungi from Nerium oleander (Apocynaceae): main constituents and antioxidante activity. World J Microbiol Biotechnol 23:1253-1263. https://doi.org/10.1007/s11274-007-9357-z
Kusari S, Hertweck C, Spiteller M (2012) Chemical ecology of endophytic fungi: origins of secondary metabolites. Chem Biol 19:792-798. https://doi.org/10.1016/j.chembiol.2012.06.004
Larena P, Sabuquillo P, Melgarejo A, De Cal J (2003) Biocontrol of Fusarium and Verticillium wilt of Tomato by Penicillium oxalicum under greenhouse and field conditions. Phytopathology 151:507-512. https://doi.org/10.1046/j.1439-0434.2003.00762.x
Leal IR, Tabarelli M, Silva JMC, Barros MLB (2003) Ecologia e conservação da caatinga. Recife:Ed. Universitária da UFPE. 822 p.
Ledwitch K, Ogburn R, Cox J, Graham R, Fritzsche A, Gosnell D, Manning T (2013) Taxol: efficacy against oral squamous cell carcinoma. Mini Rev in Med Chem 13(4):509–521. https://doi.org/10.2174/1389557511313040004
Martinez-Klimova E, Rodríguez-Peña K, Sánchez S (2017) Endophytes as sources of antibiotics. Biochem Pharmacol 134:1–17. https://doi.org/10.1016/j.bcp.2016.10.010
Mmbaga MT, Sauve RJ, Mrema FA (2008) Identification of microorganisms for biological control of powdery mildew in Cornus florida. Biol Control 44:67-72. https://doi.org/10.1016/j.biocontrol.2007.10.018
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55-63. https://doi.org/10.1016/0022-1759(83)90303-4
Nicoletti R, Buommino E, De Filippis A, Lopez-Gresa MP, Manzo E, Carella A, Petrazzuolo M, Tufano MA (2008) Bioprospecting for antagonistic Penicillium strains as a resource of new antitumor compounds. World J Microbiol Biotechnol 24:189-195. https://doi.org/10.1007/s11274-007-9455-y
Oliveira RC (2013) List of Angiosperm species of the riparian vegetation of the Apodi-Mossoró river, Rio Grande do Norte, Brazil. Check List 9:740–751. https://doi.org/10.15560/9.4.740
Pereira VM (2016) Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do quadrilátero ferrífero. 222 p. Thesis (PhD – Concentration area in Agricultural Microbiology) - Universidade Federal de Lavras. Lavras, Minas Gerais, Brazil.
Pitt JI (2000) A laboratory guide to common Penicillium species. Australia: Food Science Australia a Joint Venture of CSIRO and AFISC, 197 p.
Pitt JI, Hocking AD (1997) Fungi and food spoilage. 2nd ed. London: Blackie Academic and Professional. 540 p.
Riddell RW (1950) Permanent starned mycological preparations obtained by slide culture. Mycologia 1(42):265-270. https://doi.org/10.1080/00275514.1950.12017830
Rivera KG, Seifert KA (2011) A taxonomic and phylogenetic revision of the Penicillium sclerotiorum complex. Stud Mycol 70:139-158. https://doi.org/10.3114/sim.2011.70.03
Rodrigues, P. Santos C, Venâncio A, Lima N (2011) Species identification of Aspergillus section Flavi isolates from Portuguese almonds using phenotypic, including MALDI-TOF ICMS, and molecular approaches. J Appl Microbiol 111:877-892. https://doi.org/10.1111/j.1365-2672.2011.05116.x
Sabuquillo P, De Cal P, Elgarejo MA (2006) Biocontrol of tomato wilt by Penicillium oxalicum formulations in different crop conditions. Biol Control 37:256-265. https://doi.org/10.1016/j.biocontrol.2006.02.009
Samson RA, Frisvad JC (2004) Polyphasic taxonomy of Penicillium subgenus Penicillium: A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins. Stud Mycol 49:1-174.
Stierle A, Strobel G, Stierle D (1993) Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science, 260:214-216. https://doi.org/10.1126/science.8097061
Strobel GA, Hess WM, Ford E, Sidhu RS, Yang X (1996) Taxol from fungal endophytes and the issue of biodiversity. J Ind Microbiol Biot 17:417-423. https://doi.org/10.1007/BF01574772
Visagie CM, Houbraken J, Frisvad JC, Hong SB, Klaassen CHW, Perrone G, Seifert KA, Varga J, Yaguchi T, Samson RA (2014) Identification and nomenclature of the genus Penicillium. Stud Mycol, 78:343-371. https://doi.org/10.1016/j.simyco.2014.09.001
Wang LW, Zhang YL, Lin FC, Hu YZ, Zhang CL (2011) Natural products with antitumor activity from endophytic fungi. Mini Rev Med Chem 11:1056-1074. https://doi.org/10.2174/138955711797247716
Zeng SR, Xu QW, Ye BT, Ke Y, Fang BY, Huang XM (2005) Isolating of endophytic fungi from five medicinal plants and screening of their antibacterial activities. J Fung Res 3:24-26.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
