Sperm parameters reflect the effects of topical use of Copaifera oil (Copaifera sp.) in Wistar rats
DOI:
https://doi.org/10.70151/30h15h50Keywords:
Sperm quality, Sperm motility, Mitochondria, DNA damage, Herbal medicineAbstract
Copaiba resin oil (CO) is used in the treatment of inflammatory skin injuries in humans. This study assessed the effects of topical use of CO ointment on sperm parameters. Sixty-three Wistar male rats were anesthetized and two wounds were inflicted on the back of each rat. The healing injuries were treated daily with petroleum jelly (control group), 0.01%, and 0.1% of CO ointment until the day they were euthanized: seven, fourteen, or twenty-one days later (D7, D14, or D21, respectively). Each group contained seven animals and the traits assessed in terms of sperm quality were sperm motility, integrity of plasma membrane, mitochondrial function, DNA integrity, and histopathological characteristics. Animals treated with CO at 0.01% and 0.1% for D7 had an increase in the number of cells with injured membranes (79.6% and 68.9%, respectively) and nonfunctional mitochondria (44.8% and 48.5%, respectively) when compared to the control group (43% of injured membrane and 19% of nonfunctional mitochondria). After D14 of treatment, we observed an increase (P<0.05) in the percentage of cells with damaged DNA and injured acrosome in the Copaiba-treated groups at 0.01% (27.8% and 43.8%, DNA and acrosome respectively) and 0.1% (23.7% and 41.4%, DNA and acrosome respectively), when compared to the control group (0% and 19.2%, DNA and acrosome respectively). We concluded that the topical use of CO decreased sperm quality in the period and at the concentrations studied.
Downloads
References
Abbas MA, Taha MO, Zihlif MA, Disi AM (2013) Beta-Caryophyllene causes regression of endometrial implants in a rat model of endometriosis without affecting fertility. Eur J Pharmacol 702:12-19. 10.1016/j.ejphar.2013.01.011
Al-Alami ZM, Shraideh ZA, Taha MO (2015) β-Caryophyllene as putative male contraceptive: enhances spermatogenesis but not spermiogenesis in albino rats. Med Chem Res 24 (11):3876-3884. 10.1007/s00044-015-1428-3
Amiel E, Ofir R, Dudai N, Soloway E, Rabinsky T, Rachmilevitch S (2012) β-Caryophyllene, a compound isolated from the biblical balm of gilead (Commiphora gileadensis), is a selective apoptosis inducer for tumor cell lines. J Evid Based Complement Altern Med 2012:872394. 10.1155/2012/872394.
Brasil. Formulário de Fitoterápicos da Farmacopeia Brasileira. ANVISA: ANVISA: 126 p. 2011. https://www.gov.br/anvisa/pt-br/assuntos/farmacopeia/formulario-fitoterapico
Cal K (2006) How does the type of vehicle influence the in vitro skin absorption and elimination kinetics of terpenes? Arch Dermatol Res 297(7):311-315.
Capella S, Tillmann M, Félix A, Fontoura E, Fernandes C, Freitag R, Santos M, Félix S, Nobre M (2016) Therapeutic potential of Bixa orellana L. in skin wounds: a study in the rat model of open wound healing. Arq Bras Med Vet Zootec 68(1):104-112.
Carvalho JC, Cascon V, Possebon LS, Morimoto MS, Cardoso LG, Kaplan MA, Gilbert B (2005) Topical antiinflammatory and analgesic activities of Copaifera duckei Dwyer. Phytother Res 19(11):946-50.
Castro-e‐Silva O, Zucoloto S, Ramalho FS, Ramalho LN, Reis JM; Bastos ÁA, Brito MV (2004) Antiproliferative activity of Copaifera duckei oleoresin on liver regeneration in rats. Phytother Res 18(1):92-94.
Dahham SS, Tabana YM, Iqbal MA, Ahamed MB, Ezzat MO, Majid AS, Majid AM (2015) The anticancer, antioxidant and antimicrobial properties of the sesquiterpene β-caryophyllene from the essential oil of Aquilaria crassna. Molecules 20(7):11808-11829.
De Albuquerque KC, Da Veiga AD, Silva JV, Brigido HP, Ferreira EP, Costa EV, Marinho AM, Percario S, Dolabela MF (2017) Brazilian amazon traditional medicine and the treatment of difficult to heal Leishmaniasis wounds with Copaifera. Evid Based Complement Alternat Med 2017:8350320.
Deus R, Alves C, Arruda M (2011) Avaliação do efeito antifúngico do óleo resina e do óleo essencial de copaíba (Copaifera multijuga Hayne). Rev Bras Plantas Med 13(1):1-7.
Dias-da-silva MA, Pereira AC, Marin MC, Salgado MA (2013) The influence of topic and systemic administration of copaiba oil on the alveolar wound healing after tooth extraction in rats. J Clin Exp Dent 5(4):169-73
Dias FGG, Casemiro LA, Martins CHG, Dias LGGG, Pereira LDF, Nishimura LT, Souza FF, Honsho CS (2015) Endodontics pastes formulated with copaiba oil: action on oral microbiota and dentin bridge formation in dogs. Cien Rural 45:1073-1078.
Diefenbach AL, Muniz FWMG, Oballe HJR, Rösing CK (2018) Antimicrobial activity of copaiba oil (Copaifera ssp.) on oral pathogens: Systematic review. Phytother Res 32(4):586-596.
Du J, Dianjun G (2013) Cell cycle specificity of spermatogenic cell apoptosis in rats with experimental varicocele. Clin Lab 59(7-8):851-859.
Evenson D, Darzynkiewicz Z, Melamed M (1982) Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility. J Histochem Citochem 30(3):279-280.
Fernandes COM, Félix SR, Nobre MO (2016) Toxicidade dos fitoterápicos de interesse do SUS: uma revisão. Semin Ciênc Biol Saúde 37(1):91-104.
França LR, Ogawa T, Avarbock MR, Brinster RL, Russell LD (1998) Germ cell genotype controls cell cycle during spermatogenesis in the rat. Biol Reprod 59(6):1371-1377.
Gertsch J, Leonti M, Raduner S, Racz I, Chen JZ, Xie XQ, Altmann KH, Karsak M, Zimmer A (2008) Beta-caryophyllene is a dietary cannabinoid. Proc Natl Acad Sci USA 105(26):9099-104.
Govindarajan M, Benelli G (2016) α-Humulene and β-elemene from Syzygium zeylanicum (Myrtaceae) essential oil: highly effective and eco-friendly larvicides against Anopheles subpictus, Aedes albopictus, and Culex tritaeniorhynchus (Diptera: Culicidae). Parasitol Res 115(7):2771-2778.
Grimaldi P, Orlando P, Di Siena S, Lolicato F, Petrosino S, Bisogno T, Geremia R, Petrocellis L, Di Marzo V (2009) The endocannabinoid system and pivotal role of the CB2 receptor in mouse spermatogenesis. Proc Natl Acad Sci USA 106(27):11131-11136.
Harrison R, Vickers SE (1990). Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. J Reprod Fert 88(1):343-352.
Izumi E, Ueda-Nakamura TN, Veiga Junior VF, Pinto AC, Nakamura CV (2012) Terpenes from Copaifera demonstrated in vitro antiparasitic and synergic activity. J Med Chem 55(7):2994-3001.
Kasai T, Ogawa K, Mizuno K, Nagai S, Uchida Y, Ohta S, Fujie M, Suzuki K, Hirata S, Hoshi K (2002) Relationship between sperm mitochondrial membrane potential, sperm motility, and fertility potential. Asian J Androl 4(2):97-104.
Kawamoto T, Sueyoshi T, Zelko I, Moore R, Washburn K, Negishi M (1999) Phenobarbital-responsive nuclear translocation of the receptor CAR in induction of the CYP2B gene. Mol Cell Biol 19(9):6318-6322.
Lima SR, Junior VF, Christo HB, Pinto AC, Fernandes PD (2003). In vivo and in vitro studies on the anticancer activity of Copaifera multijuga hayne and its fractions. Phytother Res 17(9):1048-53.
Mendonça DE, Onofre SB (2009) Antimicrobial activity of the oil-resin produced by copaiba Copaifera multijuga Hayne (Leguminosae). Rev Bras Farmacogn 19(2B):577-581.
Oyeyipo IP, Skosana BT, Everson FP, Strijdom H, Du Plessis SS (2018) Highly active antiretroviral therapy alters sperm parameters and testicular antioxidant status in diet-induced obese rats. Toxicol Res 34(1):41.
Pieri FA, Mussi MC, Fiorini JE, Moreira MA, Schneedorf JM (2012) Bacteriostatic effect of copaiba oil (Copaifera officinalis) against Streptococcus mutans. Braz Dent J 23(1):36-8.
Santana SR, Bianchini-Pontuschka R, Hurtado FB, Oliveira CA, Melo LPR, Santos GJ (2014) Uso medicinal do óleo de copaíba (Copaifera sp.) por pessoas da melhor idade no município de Presidente Médici, Rondônia, Brasil. Acta Agronómica 63(4):361-366.
Silva HHG, Geris R, Rodrigues Filho E, Rocha C, Silva IG (2007) Larvicidal activity of oil-resin fractions from the Brazilian medicinal plant Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae) against Aedes aegypti (Diptera, Culicidae). Rev Soc Bras Med Trop 40:264-267.
Soares DC, Portella NA, Ramos MFS, Siani AC, Saraiva EM (2013) Trans-β-caryophyllene: An effective antileishmanial compound found in commercial copaiba oil (Copaifera spp.). Evid-Based Complement Altern Med 2013:761323.
Svetlichny G, Kulkamp-guerreiro IC, Cunha SL, Silva FE, Bueno K, Pohlmann AR, Fuentefria AM, Guterres SS (2015). Solid lipid nanoparticles containing copaiba oil and allantoin: development and role of nanoencapsulation on the antifungal activity. Pharmazie 70(3):155-64.
Tobouti PL, Martins TCA, Pereira TJ, Mussi MCM (2017) Antimicrobial activity of copaiba oil: A review and a call for further research. Biomed Pharmacother 94:93-99.
Urióstegui-Acosta M, Hernández-Ochoa I, Sánchez-Gutiérrez M, Piña-Guzmán B, Rafael-Vázquez L, Solís-Heredia M, Martínez-Aguilar G, Quintanilla-Vega B (2014) Methamidophos alters sperm function and DNA at different stages of spermatogenesis in mice. Toxicol Appl Pharmacol 279(3):391-400.
Veiga Junior VF, Rosas EC, Carvalho MV, Henriques MGMO, Pinto AC (2007) Chemical composition and anti-inflammatory activity of copaiba oils from Copaifera cearensis Huber ex Ducke, Copaifera reticulata Ducke and Copaifera multijuga Hayne - A comparative study. J Ethnopharmacol 112(2):248-254.
Wang JP, Ruan Jl, Cai Yl, Luo Q, Xu HX, Wu YX (2011) In vitro and in vivo evaluation of the wound healing properties of Siegesbeckia pubescens. J Ethnopharmacol 134(3):1033-1038.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
