Mutagenic evaluation of the aqueous extract of Jurema (Mimosa tenuiflora, Fabaceae) using the Somatic Mutation And Recombination Test (SMART) on the wings of Drosophila melanogaster
DOI:
https://doi.org/10.70151/rte1sh51Keywords:
Mimosa tenuiflora, mutagenesis, recombinogenesis, phytotherapy, Drosophila melanogasterAbstract
The Jurema, Mimosa tenuiflora, is widely used in semi-arid regions of Brazil as a remedy for an ample variety of health problems. The extract is either applied as a salve or ingested and is used as an analgesic or anti-inflammatory medicine. While the use of plants in traditional medicine is common in some populations, this practice requires detailed studies of the components of the substances used, given that even differences in dosage may have harmful effects on a person’s health. Phytochemical studies of the compounds found in some species of the genus Mimosa (Mimosa pudica and Mimosa rubicaulis) have indicated antioxidant, bactericidal, and toxicological activity, depending on the dosage, although the probable mutagenic effects are still unknown. In the present study, the possible mutagenic effects of three different concentrations of the aqueous extract of M. tenuiflora (3.91, 15.625, and 62.5 mg/ml) were evaluated using SMART (Somatic Mutation and Recombination Test) on the wings of two crosses (standard and high bio-activation) of three lineages of Drosophila melanogaster – multiple wing hairs (mwh), flare3 (flr3), and Oregon R/ flr3 (ORR/flr3). The results indicated clear mutagenic effects in all the concentrations tested, reflecting the complex phytochemical composition of the aqueous extract of M. tenuiflora including compounds that may act alone or synergistically, having direct or indirect effects on the mutagenic potential recorded in the study. A more detailed understanding of the constituents of this plant responsible for the reactions observed in this study will be necessary, however, before the therapeutic use of the extracts of this plant can be regulated more reliably.
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References
Abraham SK, Graf U (1996) Protection by coffee against somatic genotoxicity in Drosophila: role of bioactivation capacity. Food Chem Toxicol 34:1-14. https://doi.org/10.1016/0278-6915(95)00087-9
Alves ASA, Santos G C, Albuquerque UP (2018) Mimosa tenuiflora (Willd.) Poir. In: Albuquerque UP, Patil U, Máthé A (Editors) Medicinal and Aromatic Plants of South America. Dordrecht. Springer 345-353. https://doi.org/10.1007/978-94-024-1552-0_31
Akinboro A, Bakare AA (2007) Cytotoxic and genotoxic effects of aqueous extracts of five medicinal plants on Allium cepa Linn. J Ethnopharmacol 112(3):470-475. https://doi.org/10.1016/j.jep.2007.04.014
Albuquerque E, Medeiros PM de, Almeida ALS de, Monteiro JM, Lins Neto, EMF, Melo JG de, Santos, JP (2007) Medicinal plants of the caatinga (semi-arid) vegetation of NE Brazil: A quantitative approach. J Ethnopharmacol. 114:325–354.
https://doi.org/10.1016/j.jep.2007.08.017
Bezerra JJL, Pinheiro AAV, Lucena RB (2021) Phytochemistry and teratogenic potential of Mimosa tenuiflora (Willd.) Poir. (Fabaceae) in ruminants: A systematic review. Toxicon 195:78-85. https://doi.org/10.1016/j.toxicon.2021.03.010.
Bishop AJR, Schiestl RH (2002) Homologous recombination and its role in carcinogenesis. J Biomed Biotechnol. 2(2):75-85. https://doi.org/10.1155/S1110724302204052
Camargo-Ricalde SL (2000) Descripción, distribuición, anatomía, composición química y usos de Mimosa tenuiflora (Fabaceae-Mimosoideae) en México. Rev Biol Trop 48(4): 939-954. DOI 10.15517/rbt.v48i4.18985
Castro AJS, Grisolia CK, Araújo, BC de, Dias CD, Dutra ES, Nepomuceno JC (2008) Recombinogenic effects of the aqueous extract of pulp from pequi fruit (Caryocar brasiliense) on somatic cells of Drosophila melanogaster. Genet Mol Res 4:1375-1383. DOI: 10.4238/vol7-4gmr515
Chen Q, Lijun W, Lijun R (2002) Chemical studies on the constituents of Lophatherum gracile Brongn. J China Pharm Univ 19:254-259. DOI:10.3724/SP.J.1009.2009.00428
Chung Y-C, Chien C-T, Teng K-Y, Chou S-T (2005) Antioxidative and mutagenic properties of Zanthoxylum ailanthoides Sieb & Zucc. Food Chem Toxicol 43:247-252. https://doi.org/10.1016/j.foodchem.2005.05.019
Dixon DR, Pruski AM, Dixon LRJ, Jha AN (2002) Marine invertebrate eco-genotoxicology: a methodological overview. Mutagenesis 17(6):495–507. https://doi.org/10.1093/mutage/17.6.495
Dutra ES, Dias CD, Araújo BC, Castro AJS, Nepomuceno JC (2009) Effect of organic tomato (Lycopersicon esculentum) extract on the genotoxicity of doxorubicin in the Drosophila wing spot test. Genet Mol Biol 32(1):133-137. https://doi.org/10.1590/S1415-47572009005000008
Dogan EE, Yesilada E, Ozata L, Yologlu S (2005) Genotoxicity testing of four textile dyes in two crosses of Drosophila using wing somatic mutation and recombination test. Drug Chem Toxicol 28:289-301. DOI: 10.1081/dct-200064473
Dominguez XA, Garcia SG, Williams H, Ortiz C, Scott A, Reibenspies J (1989) Kukulkanins a and b, new chalcones from Mimosa tenuiflora. J Nat Prod 52(4):864-7 DOI:10.1021/np50064a035
Fonseca CA, Pereira DG (2013) Aplicação da genética toxicológica em planta com atividade medicinal. Infarma 16:7-8.
Frei H, Würgler FE (1988) Statistical methods to decide whether mutagenicity test data from Drosophila assays indicate a positive, negative or inconclusive result. Mutat Res 203:297-308. DOI: 10.1016/0165-1161(88)90019-2
Frölich A, Würgler FE (1990) Genotoxicity of ethyl carbamate in the Drosophila wing spot test: dependence on genotype-controlled metabolic capacity. Mutat Res 244:201-208. DOI: 10.1016/0165-7992(90)90129-8
Graf U, Abraham SK, Guzmán-Rincón J, Würgler F E (1998) Antigenotoxicity studies in Drosophila melanogaster. Mutat Res 402:203–209. DOI: 10.1016/s0027-5107(97)00298-4
Graf U; Spanò MA, Guzman-Rincon J, Andrade HHR (1996) The wing somatic mutation and recombination test (SMART) in Drosophila melanogaster: An efficient tool for the detection of genotoxic activity of pure compounds or complex mixtures as well as for studies on antigenotoxicity. Afr Newsl Occup Health Saf 6(1):9-13.
Graf U, Van Schaik N (1992) Improved high bioactivation cross for the wing somatic mutation and recombination test in Drosophila melanogaster. Mutat Res 271:59-67. https://doi.org/10.1016/0165-1161(92)90032-h
Graf U, Würgler FE, Katz AJ, Frei H, Juon H, Hall CB, Kale PG (1984) Somatic mutation and recombination test in Drosophila melanogaster. Environ Mutagen. 6:153-188. DOI: 10.1002/em.2860060206
Gusmán-Rincón J, Graf U (1995) Drosophila melanogaster somatic mutation and recombination test as a biomonitor. In: Butterworth FM et al. (eds). Biomonitors and biomarkers as indicators of environmental changes. Plenum Press 169-181.
Idaomar M, El-Hamss R, Bakkali F, Mezzoug N, Zhiri A, Baudoux D, Muñoz-Serrano A, Liemans V, Alonso-Moraga A (2002) Genotoxicity and antigenotoxicity of some essential oils evaluated by wing spot test of Drosophila melanogaster. Mutat Res 513:61–68. https://doi.org/10.1016/S1383-5718(01)00287-X
Kristen U (1997) Use of higher plants as screens for toxicity assessment. Toxicol in Vitro 11:181-191. https://doi.org/10.1016/S0887-2333(97)00005-2
Lindsley DL, Zimm GG (1992) The Genome of Drosophila melanogaster. Academic Press Inc, San Diego. ISBN 978-0-12-450990-0.
Martinez ST, Almeida MR, Pinto AC (2009) Alucinógenos naturais: um vôo da Europa Medieval ao Brasil. Quim Nova 32(9):2501-2507. https://doi.org/10.1590/S0100-40422009000900047
Medeiros RMT, Figueiredo APM de, Benício TMA, Dantas FPM, Riet-Correa F (2008) Teratogenicity of Mimosa tenuiflora seeds to pregnant rats. Toxicon 51:316–319. https://doi.org/10.1016/j.toxicon.2007.06.012
Nascimento MS, Paiva-Souza IO, Fernandes XA, de Moraes SZC, Araújo SS, Shan AYKV, Camargo EA, Santana AEG, Araujo BS, Estevam CS. (2016) Anti-inflammatory and antioxidant activities of the hydroethanol extract and fractions of the bark of Mimosa tenuiflora (Willd.) Poir. J Pharm Pharmacol 10(39):823-831. DOI: 10.5897/AJPP2016.4648
Ohsaki A, Yokoyama R, Miyatake H, Fukuyama Y (2006) Two diterpene rhamnosides, mimosasides B and C, from Mimosa tenuiflora. Chem Pharm Bull 54(12):1728-1729. DOI: 10.1248/cpb.54.1728
Oliveira E, Vital BR, Pimenta AS, Della Lucia RM, Ladeira AMM, Carneiro ACO (2006) Estrutura Anatômica da Madeira e Qualidade do Carvão de Mimosa tenuiflora (Willd.) Poir. Rev Árvore 30(2):311-318. https://doi.org/10.1590/S0100-67622006000200018
Pimentel LA, Riet-Correa F, Gardner D, Panter KE, Dantas AFM, Medeiros RMT, Mota, RA, Araújo JAS (2007) Mimosa tenuiflora as a cause of malformations in ruminants in the Northeastern Brazilian semiarid rangelands. Vet Pathology 44(6):928-931. DOI: 10.1354/vp.44-6-928
Raetsch, C. 2005. The Encyclopedia of Psychoactive Plants. 1a Ed. Rochester VT, Park Street Press, 326–341.
Rivera-Arce E, Gattuso M, Alvarado R, Zárate E, Agüero J, Feria I, Lozoya X (2007) Pharmacognostical studies of the plant drug Mimosae tenuiflorae cortex. J Ethnopharmacol 113:400–408. DOI: 10.1016/j.jep.2007.06.023
Rodrigues IMC, Souza Filho APS, Ferreira FA (2009) Estudo fitoquímico de Senna alata por duas metodologias. Planta daninha 27(3):507-51 https://doi.org/10.1590/S0100-83582009000300011
Romero-Jiménez M, Campos-Sánchez J, Analla M, Muñoz-Serrano, A, Alonso-Moraga A (2005) Genotoxicity and anti-genotoxicity of some traditional medicinal herbs. Mutat Res 585:147–155. DOI: 10.1016/j.mrgentox.2005.05.004
Santos JH, Graf U, Reguly ML, Andrade HHR (1999) The synergistic effects of vanillin on recombination predominate over its antimutagenic action in relation to MMC-induced lesions in somatic cells of Drosophila melanogaster. Mutat Res 444:355–365. DOI: 10.1016/s1383-5718(99)00101-1
Sinigaglia M, Lehmann M, Baumgardt P, Amaral VS, Dihl RR, Reguly ML, Andrade HHR (2006) Vanillin as a modulator agent in SMART test: Inhibition in the steps that precede N-methyl-N-nitrosourea-, N-ethyl-N-nitrosourea, ethylmethanesulphonate- and bleomycingenotoxicity. Mutat Res 607:225–230. DOI: 10.1016/j.mrgentox.2006.04.012
Sousa NC, Rezende AAA, Silva RMG, Guterres ZR, Graf U, Kerr WE, Spanó MA (2009) Modulatory effects of Tabebuia impetiginosa (Lamiales, Bignoniaceae) on doxorubicin-induced somatic mutation and recombination in Drosophila melanogaster. Genet Mol Biol 32:382-388. https://doi.org/10.1590/S1415-47572009005000042
Souza RSO, Albuquerque UP, Monteiro JM, Amorim EBC (2008) Mimosa tenuiflora [Willd.] Poir.): A review of its traditional use, phytochemistry and pharmacology. Braz Arch Biol Technol 51(5):937-947. https://doi.org/10.1590/S1516-89132008000500010
Spanó MA, Frei H, Würgle FE, Graf U (2001) Recombinogenic activity of four compounds in the standard and high biotivation crosses in Drosophila melanogaster wing spot test. Mutagenesis 16:385-394. DOI: 10.1093/mutage/16.5.385
Valadares BLB, Spanó MA (2007) Avaliação de derivados sintéticos da testosterona pelo teste de mutação e recombinação somática (SMART) em Drosophila melanogaster. 2007. 132p. Tese (Doutorado). Area de concentração: Genética e Bioquímica, Universidade Federal de Uberlândia. Uberlândia, Brasil.
Valadares BLB, Graf U, Spanó MA (2008) Inhibitory effects of water extract of propolis on doxorubicin-induced somatic mutation and recombination in Drosophila melanogaster. Food Chem Toxicol 46:1103–1110. DOI: 10.1016/j.fct.2007.11.005
Zhang Y, Zhang Yu, Wu Xiaoqin, Ren Yiping, Fu Jianyun, Zhang Ying (2004) Safety evaluation of a triterpenoid-rich extract from bamboo shavings. Food Chem Toxicol 42:1867–1875. https://doi.org/10.1016/j.fct.2004.07.005
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