Micropropagation of a brazilian species Baccharis tridentata Vahl. (Asteraceae)
DOI:
https://doi.org/10.70151/g1x14517Keywords:
Baccharis, clonal propagation, shoot tips, in vitro cultureAbstract
Baccharis tridentata Vahl is an aromatic, medicinal plant, which essential oil is constituted mainly of nerolidol and spathulenol. The aim of this study was to establish a protocol for micropropagation of this species. Shoot tips of a single greenhouse grown plant were used as explants. They were washed in running water for 3 hours, sterilized with NaOCI (1%) and inoculated in Murashige & Skoog (MS) basal medium, containing activated charcoal (1% p/v). No growth regulators were added to the medium. During the five initial days after inoculation, explants were maintained in darkness to overcome oxidation, and were cultivated at 25-27°C under 16 hour photoperiod. Half strength MS (1/2MS) and half strength B5 media were also tested. Full strength MS medium resulted in taller plants with greater values of shoot and root dry matter. Rooting was induced in the same medium, without addition of growth regulators. Acclimatization gave 80% plant survival. This protocol constitutes an efficient method that could potentially produce one thousand and five hundred (1500) homogeneous plants from a single matrix plant in a period of six months.
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