Antimicrobial Activity of Cariniana legalis (Lecythidaceae) Seedlings Subjected to Different Light Intensities
DOI:
https://doi.org/10.70151/ttb82x66Keywords:
jequitibá-rosa, aqueous extract, bacteriostatic, fungicideAbstract
Cariniana legalis, Lecythidaceae is an emergent species endemic to the Brazilian Atlantic Rainforest. Popularly known as jequitibá-rosa, this sapwood has been used in reforestation, and in folk medicine to treat leucorrhoea, female reproductive disorders and back pain. The aim of this study was to evaluate the in vitro antimicrobial activity of the aqueous extract of leaves of C. legalis seedlings subjected to different light environments, in shade houses. From the leaves, the aqueous extracts were made by means of decoction and lyophilization. The antifungal and antibacterial activity against the fungi Candida albicans, C. krusei and C. parapsilosis and the bacteria Escherichia coli and Staphylococcus aureus were evaluated. The minimum inhibitory concentration and minimum fungicidal/bactericidal concentration were determined. The results revealed that the aqueous extracts of leaves of C. legalis seedlings have bacteriostatic action for E. coli and fungicidal activity against C. krusei and C. parapsilosis. Our results suggest the antimicrobial action of anthocyanins produced under higher light radiation in the extracts of C. legalis leaves. However, further studies are needed to characterize with accuracy the substances responsible for the antimicrobial activity.
Downloads
References
Americas Regional Workshop (Conservation and Sustainable Management of Trees, Costa Rica, November 1996) (1998) Cariniana legalis. The IUCN Red List of Threatened Species 1998: e.T34747A9887065. http://dx.doi.org/10.2305/IUCN.UK.1998.RLTS.T34747A9887065.en.
Baurin N, Arnoult E, Scior T, Do QT, Bernard P (2002) Preliminary screning of some tropical plants for anti-tyrosinase activity. J Ethnopharmacol 82:155-158.
Brown AJ, Odds FC, Gow NA (2007) Infection-related gene expression in Candida albicans. Curr Opin Microbiol 10:307-313.
Carvalho DDC, Alves E, Barbosa Camargos R, Ferreira Oliveira D, Soares Scolforo JR, de Carvalho DA, Sâmia Batista TR (2011) Plant extracts to control Alternaria alternata in Murcott tangor fruits. Rev Iberoam Micol 28:173–178.
Clinical and Laboratory Standards Institute – CLSI (2002) Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard—Second Edition. CLSI document M27-A2 (ISBN 1-56238-469-4). CLSI, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA.
Clinical And Laboratory Standards Institute – CLSI (2006) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. CLSI document M7-A7. Approved Standard—Seventh Edition, which describes standard broth dilution (macrodilution and microdilution) and agar dilution techniques for measuring the in vitro susceptibility of bacteria to antimicrobial agents.
Close DC, Beadle CL (2003) The ecophysiology of foliar anthocyanin. Bot Rev The 69:149-161.
Colombo AL, Guimarães T (2003) Epidemiologia das infecções hematogênicas por Candida spp. Rev Soc Bras Med Trop 36:599-607.
Colombo AL, Júnior JNA, Guinea J (2017) Emerging multidrug-resistant Candida species. Curr Opin Infect Dis 30:528-538.
Costa GS, Dalmolin ÂC, Schilling AC, Sanches MC, Santos MS, Mielke MS (2019) Physiological and growth strategies of two Cariniana species in response to contrasting light availability. Flora 258:151427.
Fenner R, Betti AH, Mentz LA, Rates SMK (2006) Plantas utilizadas na medicina popular brasileira com potencial atividade antifúngica. Braz J Pharm Sci 42:369-394.
Gonçalves KG, Pasa MC (2015) A etnobotânica e as plantas medicinais na Comunidade Sucuri, Cuiabá, MT, Brasil. Interações 16:245-256.
Guldiken B, Ozkan G, Catalkaya G, Ceylan FD, Yalcinkaya IE, Capanoglu E (2018) Phytochemicals of herbs and spices: Health versus toxicological effects. Food Chem Toxicol 119:37-49.
Lev-Yadun S, Gould KS (2009) Role of Anthocyanins in Plant Defense. In: K. Gould et al. (eds), Anthocyanins. New York: Springer Science. 22-28.
Lima E, Teixeira De Sousa Filho P, Bastida J, Schmeda-Hirschmann G (2002) Saponins from Cariniana rubra (Lecythidaceae). Bol Soc Chil Quim 47:441-447.
Mori AS (1995) Observações sobre as espécies de Lecythidaceae do leste do Brasil. Bol Bot 14:1-31.
Orskov F, Orskov I (1992) Escherichia coli serotyping and disease in man and animals. Can J Microbiol 38:699-704.
Palomino JC, Martin A, Camacho M, Guerra H, Swings J, Portaels F (2002) Resazurin microtiter assay plate: Simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis. Antimicrob Agents Chemother 46:2710-2722.
Papon N, Courdavault V, Clastre M, Bennett RJ (2013) Emerging and Emerged Pathogenic Candida Species: Beyond the Candida albicans Paradigm. PLOS Pathog 9:1-4.
Rios J-L, Recio M (2005) Medicinal plants and antimicrobial activity. J Ethnopharmacol 100:80-84.
Rolim SG, Piotto D, Carvalho AM, Latorraca JV (2018) Crescimento e características da madeira de espécies arbóreas em experimentos silviculturais no norte do Espírito Santo. In: Rolim S and Piotto D (Eds) Silvicultura e tecnologia de espécies da Mata Atlântica. Belo Horizonte: Rona 153-159.
Sanglard D, Odds FC (2002) Resistance of Candida species to antifungal agents: molecular mechanisms and clinical consequences. Lancet Infect Dis 2:73-85.
Selvamohan T, Ramadas V, Kishore SSS (2012) Antimicrobial activity of selected medicinal plants against some selected human pathogenic bacteria. Adv Appl Sci Res 3:3374-3381.
Silva DNPB (2014) Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers. 70 p. Dissertação (Mestrado) – Universidade Federal de Mato Grosso, Cuiabá.
Silva NCC, Fernandes Júnior A (2010) Biological properties of medicinal plants: a review of their antimicrobial activity. J Venom Anim Toxins Incl Trop Dis 16:402-413.
Smith NP, Mori SA, Law W, Ribeiro M (2016) Conservation assessment of Lecythidaceae from eastern Brazil. Kew Bull 71:14.
Valladares F, Niimets U (2008) Partial sunlight tolerance, a key plant feature of complex nature and consequences. Annu Rev Ecol Evol Syst 39:237-257.
Valladares F, Garcia-Plazaola JI, Morales F, Niinemets Ü (2012) Photosynthetic responses to radiation. In: Flexas J, Loreto F, Medrano H (Eds) Terrestrial Photosynthesis in a Changing Environment: A Molecular, Physiological, and Ecological Approach. Cambridge University Press. 243-260.
Vuong C, Yeh AJ, Cheung GY, Otto M (2016) Investigational drugs to treat methicillin-resistant Staphylococcus aureus. Expert Opin Investig Drugs 25:73–93.
Zuzarte M, Vale-Silva L, Gonçalves MJ, Cavaleiro C, Vaz S, Canhoto J, Pinto E, Salgueiro L (2012) Antifungal activity of phenolicrich Lavandula multifida L. essential oil. Eur J Clin Microbiol Infect Dis 31:1359-1366.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
