Seasonal evaluation of phenylalanine ammonia-lyase activity and total phenolic and tannin contents of Byrsonima verbascifolia Rich. ex A. Juss.: a medicinal plant of Brazilian Cerrado
DOI:
https://doi.org/10.70151/qzy3vz67Keywords:
murici, Brazilian Cerrado, tannin, phenylalanine ammonia-lyase, sazonalityAbstract
An experiment was conducted, ever month, from november, 2001 to september, 2002 in order to investigate the influence of seasonal variation of physical environment on gas exchange, on leaf water state, on the phenylalanine ammonia-lyase activity and on the total phenolic and tannin contents of murici,. The used area was a praire with a physionomy of Cerrado stricto sensu, located in ljaci, south of the State of Minas Gerais. For the evaluation, plants were selected with an average height of 2.70 ± 0.21m and an average basal diameter of 0.15 ± 0.04 m. Leaf potential at 7 and 12 am and gas exchange at 10 and 12 am was evaluated ever month. The results showed that the leaf water potential was not significant seasonal variations indicating that the plants were in similar water conditions in both dry and rainy seasons. Higher photosynthetic and transpiration rates were observed during rainy season. The net photosynthesis showed higher in the morning, although no significant variations of transpiration on the evaluating hours were observed. The stomatal conductance reached similar values in both seasons, suggesting a participation of non-stomatal factors on the seasonal variation observed for the gas exchange. It was also observed significant seasonal variations on enzyme activity and total tannins contents. The increase of the phenylalanine ammonia-lyase activity and decrease of the total tannins contents were characteristic of the dry season. The levels of total phenolics had not varied significantly during the dry and rainy seasons, while the total tannin contents followed the photosynthetic response of plants, the enzyme activity was much more influenced by environmental conditions.
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ABDALA, G.C., CALDAS, L.S., HARIDASAN, M. et al. Above and belowground organic matter and root:shoot ratio in a cerrado in central Brazil. Brazilian Journal of Ecology, v.2, n. 1, p.11-23, 1998.
ABRAMS, M.D., MOSTOLLER, S.A. Gas exchange, leaf structure and nitrogen in contrasting succesional tree species growing in open and understory sites during a drought. Tree Physiology, v. 15, n.6, p.361-70, 1995.
ADÁMOLI. J., MACÉDO, J., AZEVEDO, L.G. et al. Caracterização da região dos Cerrados: In: GOEDERT, W.J. (Ed.). Solos dos cerrados: tecnologias e estratégias de manejo. São Paulo: Nobel, 1986. p.33-74.
ALMEIDA, S.P., PROENÇA, C.E.B., SANO, S.M. et al. Cerrado: espécies vegetais úteis. Planaltina: EMBRAPA-CPAC, 1998. 464p.
ASSIS, J.S., MALDONADO, R., MUNOZ, T. et al. Effect of high carbon dioxide concentration on PAL activity and phenolic contents in ripening cherimoya fruit. Postharvest Biology and Technology, v.23, n. 1, p.33-9, 2001.
ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS. Official methods of the association of official analytical chemists. 11.ed. Washington, 1970. 1015p.
BRASIL. Ministério da Agricultura e Reforma Agrária. Secretaria Nacional de Irrigação. Departamento Nacional de Meteorologia. Normas climatológicas. Brasília, 1992. 84p.
BUSTAMANTE, M.M.C., KOZOVITS, A.R., SILVA, L.F.et al. Assimilação de nitrato e taxas Fotossintéticas de três espécies lenhosas de Cerrado. In: CONGRESSO DE ECOLOGIA DO BRASIL, 3., 1996, Brasília, DF. Anais... Brasília, 1996. p.49-53.
CHAKRABORTY, U., DUTTA, S., CHAKRABORTY, B. Drougth induced biochemical changes in young tea leaves. Indian Journal of Plant Physiology, v.6, n. 1, p.103-6, 2001.
CHENG, S.H., SHEEN, J., GERRISH, C. et al. Molecular identification of phenylalanine ammonia-lyase as a substrate of a specific constitutively active Arabdopsis CDPK expressed in maize protoplasts. FEBS Letters, v.503, n.2/3, p.185-8, 2001.
DE BRUYNE, T., PIETERS, L., DEELSTRA, H. et al. Condensed vegetable tannins: biodiversity in structure and biological activities. Biochemical Systematics and Ecology, Oxford, v.27, n.4, p.445-59, 1999.
FEENY, P.P. Seasonal changes in the tannin content of oak leaves. Phytochemistry, v.7, n.5, p.871-80, 1968.
FERREIRA, D.F. Análises estatísticas por meio do Sisvar para Windows versão 4.0. In: REUNIÃO ANUAL DA REGIÃO BRASILEIRA DA SOCIEDADE INTERNACIONAL DE BIOMETRIA, 45., 2000, São Carlos. Anais... São Carlos: UFSCar/SIB, 2000. p.255-8.
FRANCO, A.C. Seasonal patterns of gas exchange, water relations and growth of Roupala montana, an evergreen savana species. Plant Ecology, v. 136, n.1, p.69-76, 1998.
FRANCO, A.C. Water and light used strategies by Cerrado woody plants. In: CONGRESSO NACIONAL DE BOTÂNICA, 51., 2000, Brasília, DF. Tópicos Atuais em Botânica. Brasília, 2000. p.292-8.
GEBAUER, R.L.E., STRAIN, B.R., REYNOLDS, J.F. The effect of elevated $CO_2$ and N availability on tissue concentrations and whole plant pools of carbon-based secondary compounds in loblolly pine (Pinus taeda). Oecologia, v.113, n.1, p.29-36, 1998.
GLYPHIS, J.P., PUTTICK, G.M. Phenolics in some southern african mediterranean shrubland plants. Phytochemistry, v.27, n.3, p.743-751, 1988.
GRAHAM, T.L., PATTERSON, B.D. Responses of plants to low, non-freezing temperatures: proteins, metabolism and acclimation. Annual Review of Plant Physiology, v.33, p.347-82, 1982.
HAGERMAN, A.E. Radial diffusion method for determining tannin in plant extracts. Journal of Chemical Ecology, v. 13, n.3, p.437-449, 1987.
HARTLEY, S., JONES, C.G., COUPER, G.C. et al. Biosynthesis of plant phenolics compounds in elevated atmospheric $CO_2$. Global Change Biology, v.6, p.497-506, 2000.
HASLAM, E. Natural polyphenols (vegetable tannins) as drugs: possible modes of action. Journal of Natural Products, v.59, n.2, p.205-15, 1996.
HATANO, T., KIRA, R., YOSHIZAKI, M.L et al. Seasonal changes in the tannins of Liquidambar formosana reflecting their biogenesis. Phytochemistry, v.25, n.12, p.2787-9, 1986.
HEYWORTH, C.J., IARSON, G.R., TEMPERTON, V. et al. The effect of elevated $CO_2$ concentration and nutrient supply on carbon-based plant secondary metabolites in Pinus sylvestris L. Oecologia, v.115, n.3, p.344-50, 1998.
HUNER, N.P.A., MAXWELL, D.P., GRAY, G.R. et al. Sensing environmental temperature change throught imbalance between energy supply and energy consumption: redox state of photosystem II. Physiologia Plantarum, v.98, n.2, p.358-4, 1996.
JANAS, K., CVIKROVÁ, M., PALAGIEWICZ, A. et al. Alterations in phenylpropanoid content in soybean roots during low temperature acclimation. Plant Physiology and Biochemistry, v.38, n.7/8, p.587-93, 2000.
JONES, D.H. Phenylalanine ammonia-lyase: regulation of its induction, and its role in plant development. Phytochemistry, v.23, n.7, p. 1349-59, 1984.
KORICHEVA, J., LARSSON, S., HAUKIOJA, E. et al. Regulation of woody plant secondary metabolism by resource availability:hypothesis testing by means of meta-analysis. Oikos, v.83, n.2, p.212-26, 1998.
KOUKI, M., MANETAS, Y. Resource availability affects differentially the levels of gallotannins and condensed tannins in Cerotonia siliqua. Biochemical Systematics and Ecology, v.30, n.7, p.631-9, 2002.
LAVOLA, A. Accumulation of flavonoids and related compounds in birch induced by UV-B irradiance. Tree Physiology, v.18, n.1, p.53-8, 1998.
LAVOLA, A., TITTO, R.J., ROSA, T.M.de la et al. Allocation of carbon to growth and secondary metabolites in birch seedlings under UV-B radiation and $CO_2$ exposure. Physiologia Plantarum, v.109, n.3, p.260-7, 2000.
MARGNA, U. Control at the level of substrate supply - an alternative in the regulation of phenylpropanoid accumulation in plant cell. Phytochemistry, v. 16, n.4, p.419-26, 1977.
MATOS, E.A., REINERT, F., MORAES, J.A.P.V. Comparasion of carbon isotope discrimination and $CO_2$ and $H_2O$ exchange between the dry and the wet season in leaves of several Cerrado woody species. Revista Brasileira de Fisiologia Vegetal, v.9, n.1, p.77-82, 1997.
MEDINA, E., FRANCISCO, M. Photosynthesis and water relations of savanna tree species differing in leaf phenology. Tree Physiology, v.14, n.12, p.1367-81, 1994.
MEINZER, F.C., GOLDSTEIN, G., FRANCO, A.C. et al. Atmospheric and hydraulic limitations on transpiration in Brazilian cerrado woody species. Functional Ecology, v.13, n.2, p.273-82, 1999.
PATAKAS, A. Diurnal changes in gas exchange and water relations in field growth grapevines. Acta Horticulturae, v.335, p.251-6, 1993.
PENG, S., SCALBERT, A., MONTIES, B. Insoluble ellagitannins in Castanea sativa e Quercus petraea woods. Phytochemistry, v.30, n.3, p.775-8, 1991.
PEREVOLOTSKY, A. Tannins in mediterranean woodland species: lack of response to browsing and thinning. Oikos, v. 71, n. 2, p. 333-40, 1994.
PIVELLO, V.R., COUTINHO, L.M. Transfer of macro-nutrients to the atmosphere during experimental burnings in an open Cerrado (Brazilian savanna). Journal of Tropical Ecology, v.8, n.4, p.487-97, 1992.
RIVERO, R.M., RUIZ, J.M., GARCIA, P.C. et al. Resistance to cold and heat stress: accumulation of phenolic compounds in tomato and watermelon plants. Plant Science, v.160, n.2, p.315-21, 2001.
ROBERTS, J.M., ROSIER, P.T.W., SRINIVASA MURTHY, K.V. Physiological studies in young eucalyptus stands in Southern India and their use in estimating forest transpiration. In: CALDER, I.R., HALL, R.L., ADLARD, P.G. (Ed.). Growth and water use of forest plantations. Chichester: John Wiley & Sons, 1992. p.226-43.
RODRIGUES, V.E.G.; CARVALHO, D.A. Plantas medicinais no domínio dos cerrados. Lavras: UFLA, 2001. 180p.
SALTVEIT, M.E. Wound induced changes in phenolic metabolism and tissue browing are altered by heat shock. Postharvest Biology and Technology, v.21, n.1, p.61-9, 2000.
SANTOS, S., PALAZZO DE MELLO, J.C. Taninos. In: SIMÕES, C.M.O. Farmacognosia: da planta ao medicamento. 2. ed. Porto Alegre: Ed. Universidade, 2000. p.517-44.
SARMIENTO, G. The ecology of neotropical savannas. Cambridge: Harvard University Press, 1983. 235p.
SCOOTT, A.J., KNOTT, M.A cluster analysis method for grouping means in the analysis of variance. Biometrics, v.30, n.3, p.507-12, 1974.
SOLECKA, D., BOUDET, A.M., KACPERSKA, A. Phenylpropanoid and anthocyanin changes in low-temperature treated winter oilseed rape leaves. Plant Physiology Biochemistry, v.37, n.6, p.491-6, 1999.
SOLECKA, D., KACPERSKA, A. Phenylalanine ammonia-lyase in leaves of winter oilseed rape plants as affected by acclimation of plants to low temperature. Plant Physiology and Biochemistry, Montrouge, v.33, n.5, p.585-91, 1995.
SOUZA, C.R. Alguns aspectos da enxertia de mesa com forçagem e respostas fisiológicas à deficiência hídrica na produção de mudas de videira. 1999. 75f. Dissertação (Mestrado em Fisiologia Vegetal) - Universidade Federal de Lavras, Lavras, MG.
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