Genetic diversity in a jaborandi (Pilocarpus microphyllus Stapf.) germplasm bank assessed by RAPD markers
DOI:
https://doi.org/10.70151/7mes1852Keywords:
molecular markers, medicinal plants, AMOVA, genetic markersAbstract
The aim of this work is to assess the occurrence of genetic variability in a P. microphyllus germplasm bank from Embrapa Amazonia Oriental in Belém, PA, using RAPD markers. DNA was extracted from 93 plants, belonging to 12 sample areas owned by the P. microphyllus germplasm bank. Dendrograms were constructed using Nei & Li's genetic similarity coefficient among plants and among sample areas using NTSYS-pc program. To verify variability distribution, an analysis of molecular variance (AMOVA) was obtained, generating among and within sample areas variance. The Shannon index was used to verify the genetic diversity of each area. It was obtained a fine amplitude of similarities among individuals, but the dendrogram did not show a complete clustering of the individuals according to their origin. AMOVA obtained 24.16% among areas variation and 75.84% within areas.
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