Antifungal activity of Eugenia uniflora L. fractions against Paracoccidioides brasiliensis (Splendore) Almeida

Authors

  • S. C. Santos Universidade Federal de Goiás
  • J. P. Ribeiro Universidade Federal de Goiás
  • D. O. Guimarães Universidade Federal de Goiás
  • M. O. Silva Universidade Federal de Goiás
  • P. H. Ferri Universidade Federal de Goiás
  • A. C. F. Garcia Universidade Federal de Goiás
  • J. S. Pires Universidade Federal de Goiás
  • A. C. M. Castro Universidade Federal de Goiás
  • M. R. R. Silva Universidade Federal de Goiás
  • J. R. Paula Universidade Federal de Goiás

DOI:

https://doi.org/10.70151/kw55zz76

Keywords:

Eugenia uniflora, pitangueira, Paracoccidioides brasiliensis, sesquiterpenes, plants medicinal

Abstract

The Bioassay-directed fractionation has investigated the activity of E. uniflora leaves crude extract against the dimorphic fungus Paracoccidioides brasiliensis using the agar dilution method. The ethanolic crude extract inhibited the fungal growth at 750 mg.mL⁻¹. Fractionation by partition between solvents yielded three fractions. The ether fraction showed the best activity with minimal inhibitory concentration (MIC) at 187.5 mg.mL⁻¹. This fraction was refractionated by vacuum liquid chromatography, affording four sub-fractions. F1 and F2 achieved MIC at 375 mg.mL⁻¹, while F3 MIC at 500 mg.mL⁻¹. GC-MS analysis of F1 and F2 have indicated the presence of mainly oxygenated sesquiterpenes. TLC analysis of F3 and F4 (inactive), suggested the presence of terpenoids and flavonoids for the first and only flavonoids for the later. The chemical constituents of the ether fraction are terpenoids, mainly oxygenated sesquiterpenes, which probably are the responsible for the antifungal activity. Furthermore, the reduction in the antifungal activity indicated a synergism among the bioactive compounds.

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Author Biographies

  • S. C. Santos, Universidade Federal de Goiás

    Laboratório de Bioatividade Molecular

  • J. P. Ribeiro, Universidade Federal de Goiás

    Laboratório de Bioatividade Molecular

  • D. O. Guimarães, Universidade Federal de Goiás

    Laboratório de Bioatividade Molecular

  • M. O. Silva, Universidade Federal de Goiás

    Laboratório de Bioatividade Molecular

  • P. H. Ferri, Universidade Federal de Goiás

    Laboratório de Bioatividade Mocelular

  • A. C. F. Garcia, Universidade Federal de Goiás

    Laboratório de Micologia

  • J. S. Pires, Universidade Federal de Goiás

    Laboratório de Micologia

  • A. C. M. Castro, Universidade Federal de Goiás

    Laboratório de Micologia

  • M. R. R. Silva, Universidade Federal de Goiás

    Laboratório de Micologia

  • J. R. Paula, Universidade Federal de Goiás

    Laboratório de Farmacognosia

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Published

2004-06-04

Issue

Vol. 7 No. 1 (2004)

Section

Articles

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How to Cite

Antifungal activity of Eugenia uniflora L. fractions against Paracoccidioides brasiliensis (Splendore) Almeida. (2004). Revista Brasileira De Plantas Medicinais, 7(1), 30-33. https://doi.org/10.70151/kw55zz76