Foliar anatomy with emphasis in the secretory trichomes and chromatographic analysis of the essential oil of Melissa officinalis L. (Lamiaceae)

Authors

  • M. B. G. Martins Universidade Estadual Paulista
  • A. P. Pastori Universidade Estadual Paulista

DOI:

https://doi.org/10.70151/yb0pd215

Keywords:

Melissa officinalis, secretory trichomes, foliar anatomy, monoterpenes, volatile oils

Abstract

Adult leaves of Melissa officinalis L. (Lamiaceae) harvested during the time of budding had been used in the study of the leaf anatomy in the identification of the secretory structures. The material was fixed in FAA 50%, dehydrated in alcoholic series (ethyl), infiltrated in paraffin, sectioned at 13 mm, staining and later analyzed through optic microscopy. Also it was carried through a study in gaseous chromatography for attainment of the crude essential oil. Analyses of transversal sections of the leaf of Melissa officinalis, has identified the presence of two types of secretory trichomes: peltate and capitate, beyond the presence of trichome tector. Trichome capitate, identified in literature as (type 1), presents variations in its morphology in relation to the cells number stalk and the head secretory cells number. The chromatographic analysis of the essential oil identified the presence of monoterpenes, in two major components, responsible for more than 87.8% of the relative composition in the crude oil, beyond sesquiterpenes in smaller proportions.

Downloads

Download data is not yet available.

References

ADAMS, R.P. Identification of essential oil by lon trap mass spectroscopy. San Diego: Academic Press, 1995. p.469.

ADZET, T., PONZ, R., WOLF, E. et al. Content and Composition of M. officinalis oil in relation to leaf position and harvest time. Planta Medica, v.58, p.562-4, 1992.

AMELUNXEN, F. Elektronenmikroskopische Untersuchungen na den Drüsenschuppen von Mentha piperita L. Planta Medica, v.13, p.457-73, 1965.

ASCENSÃO, L., MARQUES, N., PAIS, M. S. Glandular trichomes on vegetative and reproductive organs of Leonotis leonurus (Lamiaceae). Annals of Botany, v. 75, p.619-26, 1995.

ASCENÇÃO, L., FIGUEIREDO, A. C., BARROSO, J. G. et al. Plectranthus madagascariensis: Morphology of the glandular trichomes, essential oil composition, and its biological activity. Internacional Journal of Plant Science, v.159, n.1, p.31-8. 1998.

CARNAT, A.P., CARNAT, A., FRAISSE, D. et al. The aromatic and polyphenolic composition of lemon balm (Melissa officinalis L. subspécie officinalis) tea. Pharmaceutica Acta Helvetiae, v. 72, p. 301-5,1998.

FAHN, A. Secretory tissues in plants. London: Academic Press, 1979. p. 158-222.

HEINRICH, G. Entwicklung, Feinbau und ölgehalt der drüsenschuppen von Monarda fistulosa. Planta Medica, v.23, p. 154-66, 1973.

HERTWIG, I.F.V. Plantas aromáticas e medicinais plantio, colheita, secagem, comercialização. São Paulo: Ícone. 1986. p.331-3.

JOHANSEN, D.A. Plant microtechnique. New York: McGraw-Hill, 1940. p.523.

LADEIRA, A. M. Plantas medicinais. São Paulo: Instituto de Botânica, 1983. p.21-2. (folheto n.15).

LAWRENCE, B.M. Essential oils: 1976-1978, Wheaton: Allured Publ. Co. 1979. v.1.

MARTINS, E.H., CASTRO, D.M., CASTELLANI, D.C. et al. Plantas medicinais. Viçosa: UFV, 1998. p.114-5.

McMURRY, J. Química Orgânica. 4.ed. Rio de Janeiro: Livros técnicos e científicos. 1997. p.469-71.

NAVARRO, T., EL QUALIDID, J. Trichome morphology in Teucrium L. (Labiatae). A taxonomic Review. Anales Jardim Botanic Madrid, v.57, n.2, p.277-97, 2000.

SARER, E., KÖKDIL, G. Constituents of the Essential Oil from Melissa officinalis. Planta Medica, v.57, p.89-90, 1991.

SCHNEPF, E. Tubulares endoplasmatisches reticulum in Drüsen mit lipophilen Ausscheidung von Ficus, Ledum un Salvia. Biochemistry Physiology Pflanzenphysiology, v. 163, p. 113-25, 1972.

SIMÕES, C. M. O., SPITZER, V. Óleos voláteis. In: Farmacognosia: da planta ao medicamento. 2. ed. Porto Alegre e Florianópolis: Editora da UFRGS e UFSC, 2000. p.387-415.

TURNER, G.W., GERSHENZON, J., NIELSON, E.E. et al. Limonene synthase, the enzyme responsible for monoterpene biosynthesis in peppermint, is localized to leucoplasts of oil gland secretory cells. Plant Physiology, v. 120, p.879-86, 1999.

WERKER, E., RAVID, U., PUTIEVSKY, E. Structure of glandular hairs and identification of the main components of their secreted material in some species of the Labiatae. Israel Journal of Botany, v.34, p.31-45, 1985.

WERKER, E. Function of essential oil-secreting glandular hairs in aromatic plants of the Lamiaceae a review. Flavour and Fragance Journal, v.8, p.249-55, 1993.

Published

2003-12-08

Issue

Section

Articles

How to Cite

Foliar anatomy with emphasis in the secretory trichomes and chromatographic analysis of the essential oil of Melissa officinalis L. (Lamiaceae). (2003). Revista Brasileira De Plantas Medicinais, 6(2), 77-82. https://doi.org/10.70151/yb0pd215