Acute toxicity (LD₅₀) determination of the Simarouba versicolor, St. Hill, aqueous extract in mice
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https://doi.org/10.70151/zhxetg30Keywords:
Simarouba versicolor, Acute Toxicity, Mice and LD₅₀Abstract
In popular medicine, several plants have been used for treatment of the most varied illnesses, however these plants are used without any scientific criterion or test. The Simarouba versicolor is known popularly as "pau-paraíba", asher rind and fruits are used as anthelminthic, against snake poison, diarrhea, dysentery, dyspepsia, fever and hemorrhage. This research was developed to determine the acute toxicity in mice (LD₅₀) of the aqueous extract of the S. versicolor rind. The botanical material was collected in Sept/2000 in municipal district of Angical - Pl and identified in the "Herbário Graziela Barroso/UFPI" exsiccate number 14.301/TEPB. The rind was dried in oven and later ground in mill. The aqueous extract was prepared in distilled water with the concentration of 10% (m/v) and submitted to broil two minutes out of 100 and were used twenty adult mice, with weight of 26 g and divided in groups containing males and females in equal number, to which were administered through probe intragastric 0,1 mL for 10 g of body weight (BW) of the aqueous extract and of the physiologic solution (control group), the same volume was administered by the intraperitoneal route (IP), being computed the number of deaths soon afterwards during the period of 72 hours. After determination of dry weight was calculated (mg/kg), the administered doses by IP and VO way, which were 16; 32; 64; 128; 256 and 88.5; 177; 260.4; 356.4 and 433.7 mg/Kg, respectively. The Lethal Average Dose (LD₅₀) was calculated through probitos using the program SPSS for windows version 8.0/UFPI, and obtained the following values: 68.80 and 185.88 mg/kg with superior trust limits and inferior to 95,96-49.27 and 29.20-134.81 mg/kg, respectively. This study reveals that the values made calculations for LD₅₀ which suggest a toxic potential activity from extract. Also, it was observed that LD₅₀= 68.80, determinated for IP route is approximately a third of the dose administered by VO.
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