Bromelian enzyme activity on pineapple plants (Ananas comosus L. Merril) under saline conditions "in vitro"
DOI:
https://doi.org/10.70151/k7t56z11Keywords:
Pineapple, bromelians, enzyme activators, saltpetre soilsAbstract
Bromelian enzyme activity on pineapple plants (Ananas comosus L. Merril) under saline conditions "in vitro". The objectives of this work were to characterize pineapple plants (Ananas comosus L. Merril) cv. Smooth Cayenne, cultured "in vitro", in saline medium, in relation to bromelian activity, identifying the parts of the plant with the highest bromelian activity. Also under aim was the study of the influence of saline stress on the enzyme activity. Axillary buds of pineapple were cultivated "in vitro" in MS medium, supplemented with 2 mg.L-1 BAP and 1 mg.L-1 NAA. The levels of salinity tested were: 0.57 g.L-1 NaCl, 1.15 g.L-1 NaCl, and 2.30 g.L-1 NaCl. Bromelian activity was evaluated in the development of buds, shoots, and roots. The results showed that bromelian activity was higher in buds at the highest salt concentration at 15 days. Cultured shoots showed bromelian activity decreasing in the saline treatments in all the collection, up to 60 days in culture. The roots showed higher bromelian activity in the roots in saline medium.
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