Toxic and mutagenic activity of oleoresin of Copaiba (Copaifera langsdorfii Desfon) in mice
DOI:
https://doi.org/10.70151/z6gvgb44Keywords:
mutagenicity tests, micronuclei, mice, medicinal plantsAbstract
The oleoresin of Copaiba, Copaifera langsdorfii Desfon, which has been used by the Brazilian population for treatment of cancer, ulcer, syphilis, bronchitis, and diarrhea, showed a potent anti-tumor activity against IMC carcinoma in mice. In this present study, the aim was to evaluate the mutagenic activity and the cyto-toxicity of the oleoresin of "copaiba" in the mouse, with the bone marrow micronucleus test. Doses of oleoresin of 2.11ml/kg (25% of LD50), 4.22mL/kg (50% of LD50), and 6.76mL/kg (80% of LD50) were given by oral administration to mice Mus musculus in groups of 7 animals for each treatment. For all the doses, micronucleated polychromatic erythrocytes frequency (MNPCE) was evaluated at periods of 24, 48, and 72 hours. Cytoxicity was evaluated polychromatic and normo-chromatic erythrocytes ratio (PCE and NCE). Results showed that the oleoresin in doses of 4.22 mL/kg, (50% of LD50) and 6.76mL/kg (80% of LD50) increased significantly (p<0.001) the frequency of MNPCE compared to negative control group. In relation to the administered time, the maximum frequency of induction of MNPCE was observed in 24h. These results indicate that the oleoresin of "copalba" exhibited mutagenic activity, Cytotoxicity was evident in all dosages applied.
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