Evaluation of different systems of "in vitro" culture for propagation of Psychotria ipecacuanha (Brot.) Stokes
DOI:
https://doi.org/10.70151/4zxjcj15Keywords:
ipecac, tissue culture, medicinal plants, Rubiaceae, alkaloids, ipecacuanhaAbstract
This work was carried out with Psychotria ipecacuanha, a Brazilian medicinal plant the roots of which contain emetine. The main objective was to develop a protocol for the micro-propagation of these species, by testing different culture techniques, the temporary immersion system, and the semi-solid and liquid media systems. In the semi-solid system, experiments were developed in flasks of two different sizes containing MS, B5, and WP media to which were added different growth regulators. Innoculum density was also evaluated. The liquid medium system consisted of MS medium supplemented with different growth regulators. For the temporary immersion system, the MS medium received an addition of 1.5mg/L BAP and 0.5mg/LGA3 and a reverse digital apparatus and vacuum pump were used. The liquid medium system with MS medium supplemented with 1.5mg/L BAP and 0.5mg/LGA3, presented the best results for shoot proliferation in a period of 30 days in culture (2.37±0.32 shoots/explant). Cultures carried out for 90 days in the semi-solid system, using 8.5 x 5.5cm flasks and 3 explants per flask, developed 1.80 ± 0.20 shoots /explant, achieving 3.06±0.51 cm of height adn presented superior survival ratio (96%). Explants cultured in temporary immersion system for 90 days showed 2.30±1.10 shoots/explant achieving a growth of 2.08±0.12 cm and 52% survival.
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ADELBERG, J.W., DESAMERO, N.V., HALE, S.A. et al. Long-term nutrient and water utilization during micropropagation of Cattleya on a liquid / membrane system. Plant Cell, Tissue and Organ Culture, v.48, p.1-7, 1997.
ALVARD, D., COTE, F., TEISSON, C. Comparison of methods of liquid medium culture for banana micropropagation. Plant Cell, Tissue and Organ Culture, v.32, . 55-60, 1993.
CABASSON, C., ALVARD, D., DAMBIER, D. et al. Improvement of Citrus somatic embryo development by temporary immersion. Plant Cell, Tissue and Organ Culture, v.50, p.33-7, 1997.
RODRIGUES, I.A., ALVES, S.M., NETO, G.R. et al. Geração de tecnologia agroindustrial para o desenvolvimento do trópico úmido, Belém-PA. Brasília: EMBRAPA-CPATU/JICA, 1996. p.240-6.
DIGITAL REVERSE SMR 07. LABTEC EQUIPAMENTOS PARA LABORATÓRIOS LTDA. Departamento de Química, Universidade de Ribeirão Preto.
FUNDAÇÃO BIODIVERSITAS. Disponível em:
GAMBORG, O.L., MILLER, R.A., OJIMA, K. Nutrient requeriments of suspension cultures of soybean roots cells. Experimental Cell Research, v.50, p.151-8, 1968.
GRATTAPAGLIA, D., MACHADO, M.A. Micropropagação. In:. TORRES, A.C., CALDAS, L..S., BUSO, J.A. (Ed.). Cultura de tecidos e transformação de plantas. Brasília: Embrapa SPI, 1998. 864p.
IDEDA, K., TESHIMA, D., AOYAMA, T. et al. Clonal propagation of Cephaelis ipecacuanha. Plant Cell Reports, v.7, p.288-91, 1988.
JHA, S., JHA, T.B. Micropropagation of Cephaelis ipecacuanha Rich. Plant Cell Reports, v.8, p. 437-9, 1989.
LAMEIRA, O.A., COSTA, M.P., PINTO, J.E.B.P. The efficiency of shoot and plantlet formation of Cephaelis ipecacuanha after three subcultures "in vitro". Ciência Rural, Santa Maria, v.24, n.3, p.523-6, 1994.
LLOYD, G., McCOWN, B. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Combined Proceedings International Plant Propagators' Society, v.30, p.421-7, 1980.
MACKAY, W.A., KITTO, S.L. Factors affecting in vitro shoot proliferation of French Tarragon. Journal of the American Society Horticulturae Science, v.113, n.2, p.282-7, 1988.
MURASHIGE, I., SKOOG, F. A revised medium for rapid growth and bio assays with tabacco tissue cultures. Physiologia Plantarum, v. 15, p.473-97, 1962.
PEREIRA, A.M.S., BERTONI, B.W., PEREIRA, P.S. et al. The effect of "in vitro" co-cultivation of Cephaelis Ipecacuanha, Eclipta alba and Oryza sativa on plant development and yield of emetine, wedelolactone and demethylwedelolactone. Acta Horticulturae, v.502, p.307-11, 1999.
PEREIRA, J.E.S., FORTES, G.R.L., SILVEIRA, A.O. Efeito da densidade de inoculação na multiplicação "in vitro" da batata. Horticultura Brasileira, v. 18, supl. jul., 2000.
PÉREZ PONCE, J.N., GONZÁLEZ, E.A.J., PEÑALVER, D.A. Aumento de la eficiencia en la micropropagación. In: PÉREZ PONCE, J.N. (Ed.). Propagación y mejora genética de plantas por biotecnología. Santa Clara: Instituto de Biotecnología de las Plantas, 1988. 400р.
TREASE, G.E., EVANS, W.C. Pharmacognosy. 13.ed. London: Bailliére Tindal, 1989. p.595-9.
VASIL, I.K. Automation of plant propagation. Plant Cell, Tissue and Organ Culture, v.39, p. 105-8, 1994.
YOSHIMATSU, K., SHIMOMURA, K. Efficient shoot formation on internodal segments and alkaloid formation in the regenerates of Cephaelis ipecacuanha A. Richard. Plant Cell Reports, v.9, p.567-70, 1991.
YOSHIMATSU, K., SHIMOMURA, K. Plant regeneration on cultured roots segments of Cephaelis ipecacuanha A. Richard. Plant Cell Reports, v.14, p.98-101, 1994.
YOSHIMATSU, K., AOI, K., SHIMOMURA, K. Clonal propagation of Cephaelis ipecacuanha (II): characteristics of regenerated plants field-cultivated in two districts. Journal of Plant Physiology, v.144, p.22-5, 1994.
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