A comparison of thin layer chromatography and high performance liquid chromatography for artemisinin analyses
DOI:
https://doi.org/10.70151/ewq7st24Keywords:
artemisinin, Artemisia annua L., densitometry, thin layer chromatography, high pressure liquid chromatographyAbstract
Artemisia annua L, a native of China and adapted Io Brazilian climate, is a rich source of artemisinin, a sesquiterpene lactone proven to be effective against the malaria agent Plasmodium. The scope of this work was to compare the method of thin layer chromatography (TLC) using densitometnc detection with high performance liquid chromatography (HPLC) using ultraviolet detection for the analytical quantification ol the artemisinin contents. The results show that the TLC method does not have the same selectivity and sensibility necessary to be a useful alternative method to HPLC. Under experimental conditions the TLC method over-estimated the artemisinin contents, compared to those determined by HPLC.
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ACTON, N . KLAYMAN. DL., ROLLMAN, I J Reductive eletrochemical HPLC assay for artemisinin (qinghaosu) Planta Medica, v,5. p 445, 1985.
BRYSON. C.T.. CROOM JUNIOR, E M. Herbicide imputs for a new agronomic crop, annual wormwood (Artemisia annua) Weed Technology, v.5, p 117-24, 1991.
BUTLER, D . MAURICE, J., O BRIEN. C Time to put malana control on the global agenda Nature, v 386, p.535-541. 1997.
CHARLES. D.J., SIMON. J.E . WOOD. K.V.. HEINSTEIN, P. Germplasm variation in artemisinin content of Artemisia annua using an alternative method of artemisinin analysis from crude plant extracts Journal of Natural Products. v53. n.1, p.157’60, 1990.
DELABAYS. N Biologie de la reproduction chez L*Artemisia annua L. et genetique de la production en artemisinine. Lausanne. 1997 I69p. Tese (Doutorado) - Umversité de Lausanne DELABAYS, N., JENELTEN, U . PARIS. M . PIVOT. D.. GALLAND. N. Aspects agronomiques et génétiques de la production dartémismine a partir Artemisia annua L Revue Suisse de Viticulture Arboriculture Horticulture, v.26, p.291 -296. 1994.
ELHAG, H.M . EL-DOMIATY. MM. EL-FERALY. F.S.. MOSSA. J.S. & EL-OLEMY, M M Selection and micropropagation of high artemisinin producing clones of Artemisia annua L Phytotherapy Research. v6. p 20-4. 1992.
ELSOHLY, H.N . CROOM. E M . ELSOHLY. M A Analysis of the antimalarial sesquiterpene artemisinin in Artemisia annua by High-Performance Liquid Chromatography (HPLC) with postcolumn derivatization and ultraviolet detection Pharmaceutical Research, v.4, n.3, p 258-60, 1987.
FERREIRA. J.F.S Production and detection of Artemisinin in Artemisia annua L. Indianapolis, 1994, 125p Tese (Doutorado) - Purdue University FERREIRA. J.F.S.. JANICK, J. Immunoquantitative analysis of artemisin from Artemisia using plyclonal antibodies Phytochmnistry, v41, n.1, p.97-104, 1996.
FERREIRA. J P S., CHARLES, D.J.. WOOD. K., JANICK, J., SIMON, J.E. A comparison of gas chromatography and high performance liquid chromatography for artemisinin analyses Phytochemical Analysis, v.5. p 116-20, 1994.
FOGLIO. M.A ,estudo químico da Artemisia annua L. aclimatada no Brasil, Campinas. 1996 169p Tese (Doutorado). Instituto de Química. Universidade Estadual de Campinas.
GELDRE. E.V., VERGAUWE. A., EEKHOUT. E.V. State of the art of the production of the antimalarial compound artemisinin in plant. Plant Molecular Biology, v.33, p.199-209, 1997.
GUPTA, M.M., JAIN, D,C„ VEMIA, R.K., GUPTA. A.P. A rapid analytical method for the estimation of artemisinin in Artemisia annua. Journal of Medicinal and Aromatic Plant Sciences, v.18, p.5-0. 1996.
JAZIRI. M. DIALLO, B , VANHAELEN, M.. HOMES, J., YOSHIMATSU, K., SHIMOMURA. K. Immunodetection of artemisinin in Artemisia annua in cultivated hydroponic conditions. Phytochemistry, v.33, n.4, p.821-6, 1993.
KLAYMAN, D.L. Qinghaosu (artemisinin); an antimalariai drug from China Science, v_228, p. 1049-55, 1985.
LIERSCH, R.. SOICKE, H., STEHR, C„ TÜLLNER, H.U. Formation of artemisinin in Artem/wa annua during one vegetation period. Planta Medlca, v.52. p.387-90. 1986.
LOPES, J.LC. Cromatografia em camada delgada. In: Collins, G.L Braga , G.L. e Bonato, PS. coord. Introdução a métodos cromatográficos 61 ed., Campinas: Editora da UNICAMP, 1995. 45-56.
MAGALHÃES, P M Seleção, melhoramento e nutrição da Artermsia annua L., para cultivo em região IntertropicaL Campinas, 1996. il7p,Tese (Doutorado) - Instituto de Biologia, Universidade Estadual de Campinas.
MAGALHÃES, P.M , DELABAYS, N , SARTORATTO, A. New hybrid lines of the antimalariai species Artemisia annua L guarantee its growth in Brazil. Ciência e Cultura, v.49r n.5/6. p.413-5, 1997.
MARCHESE, J A. Produção e detecção de artemisinins em plantas de Artemisia annua L. submetidas a estresses abióticos. Campinas, 1999 8Bp. Tese (Mestrado) - Universidade Estadual de Campinas.
PRAS. N., VISSER, J.F.r BATTERMAN, S. WOERDENBAG. HJ. & MALINGRÉ, T,M. Laboratory selection of Artemisia annua L. for high artemisinin yelding types. Phytochemical Analysis, v.2, p.80-3, 1991.
SIPAHIMALANI. A.T., FULZELE, D.P., HEBLE.M.R. Rapid method for the detection and determination of artemisinin by gas chromatography. Journal of Chromatography, v.538, p.452-5, 1991.
WHO (World Health Organization), 1998. Malaria." Fact Sheet n* 94, 10/1998. Disponível emxhttp;// www.who.int/inffs/en/fact094.html> Acesso (01/09/ 2000).
WOERDENBAG. J.H.. PRAS, N.r BOS, R., VISSER. J.F.. HENDRIKS, H. & MALINGRÉ, TM Analysis of artemisinin and related sesquilerpenoids from chromatography/mass Artemisia annua L. by combined gas spectrometry. Phylochemlcal Analysis, v.2, p.215-9. 1991.
ZHAO. S. , ZENG, M.Y. Aplication of precolumn reaction to high-performance liquid chromatography of qinghaosu in animal plasma. Analytical Chemistry, v.58. p.289-92, 1986.
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