Droplet counter-current chromatography in the separation of phenolic substances from Neuropogon auraníteco-ater
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https://doi.org/10.70151/yqgx7208Keywords:
Chromatography, countercurrent distribution, lichens, phenolsAbstract
This work reports the separation of phenolic compounds present in the metanolic extract of the lichen Neuropogon aurantiaco-ater (Usmiaceae). The crude extract was fractionated by droplet counter-current chromatography using a solvent mixture of high polarity (chloroform:methanol:water at 43:37:20, in ascending mode). The separation resulted in the isolation of usnic and protocetratic acid, which were identified by TLC, HPLC, and NMR tests.
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