Micropropagation of Salix humboldtiana Hild.
DOI:
https://doi.org/10.70151/ea5tbg97Keywords:
Tissue culture, Salix humboldtiana Hild., salgueiro, medicinal plantsAbstract
A micropropagation protocol for large scale production of Salix humboldtiana Hild. was carried out using both, explants excised from adult plants and from cloned plantlets. Either shoot proliferation or root induction were influenced by the source of explant used. MS medium supplemented with 2,3 μM kinetin enhanced shoot production while half-strength MS medium added with 5.3 μM NAA enhanced rooting. During the acclimatization process, irrigation with 0.005% (v/v) ampicillin solution was essential for plant maintenance.
Downloads
References
GRÔNROOS, L„ HARDNER, C.. GULLBER, U. Field performance of Salix clono propagated via shoot culture in vitro. Scandinavian Journal of Forest Research, v. 5. p. 487-92, 1990.
GUPTA, M P. 270 plantas medicinales Iberoamericanas. Santafé de Bogotá: Presencia. p.495, 1995.
HAUTH. S., BEIDERBECK, R. In vitro culture of agrobacterium rhizogenes-induced hairy roots of Salix alba L. Silvae Genetics ,v.41, n 1. p.468, 1991.
LINEBERGER, R.D. Shoot proliferation, rooting, and transplant survival of tissue cultured Hally jolivette cherry. Hortscience, v. 18, p. 182-5, 1983.
USKOVA, D., ZAKUTNÁ, L„ KÁKONIOVÁ, D. Pigment formation in Willow tissue culture. Biologia (Bratislava) ,v.44 , n.11, p. 1039-45, 1989.
MURASHIGE, T, SKOOG, F. A revised medium for rapid growth and bio assays with tabacco tissue cultures. Physiologia Plantarum v.15, p.473-97, 1962.
NEUNER, H., BEIDERBECK, R. In vitro propagation of Salix caprea L. single node explant. Silvae Genetica , v.42, n.6, p.308-10. 1993.
PEZZUTO, J.M., CHE. C.T., McPHERSON. D.D., ZHU, J.P., TOPCU, G., ERDELMEIER, C A.J , CORDELL, G.A. DNA as an affinity probe useful in the detection and isolation of biologically active natural products. Journal of Natural Products, v.54, n.6. p. 1522-30. 1991.
SKIRVIN, R.M., CHU. M.C . RUKAN, H. Tissue culture of peach, sweet and sour cherry, and apricot shoot tips. Proceedings of State Horticultural Society, v.113. p.30-8, 1980.
STOEHR, M.U., MANTANG. C., ZSUFFA. L In vitro plant regeneration via callus culture of mature Salix exigua. Canadian Journal of Forest Research, v.19, p. 1634-8. 1989.
VAHALA, T, ERIKSSON, T. Callus production from Wilow (Salix viminalis L.) protoplasts. Plant Cell Tissue and Organ Culture, v.27, p.243-8,1991.
Downloads
Published
Issue
Section
License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.