The treatment with Caesalpinia peltophoroides extract fractions in mice: mutagenic, antimutagenic and metabolic evaluation
DOI:
https://doi.org/10.70151/c4maw071Keywords:
Caesalpinia peltophoroides, micronuclei, toxicity, metabolismoAbstract
The aim of this study was to evaluate the methanol and ethyl acetate fraction of leaf and flower of Caesalpinia peltophoroides as mutagenic and antimutagenic ability, and metabolic effects. Sixty Swiss mice were divided into ten groups with n=6 each. The mice were weighed at the beginning and end of treatment and daily received orally 150 mg/kg of ethyl acetate fraction of flower (AFL) or leaf (AFO); or else methanolic fraction of flower (MFL) or leaf (MFO), or water for 15 consecutive days. On the 15th day, the mice were divided half in each group to receive saline (S) or 25 mg/kg of cyclophosphamide (CPA), and there was also the presence of positive and negative control groups (no plant fraction, only S or CPA, respectively). After 24 h, the mice were killed, and the biological material were collected. The plant fractions were analyzed by liquid chromatography method, in which all fractions characterized as violet spectrum. The frequency of micronuclei compared to the positive control group was 60.1% for AFL, 55% for MFO, 51.7% for AFO and 48.3% for MFL. There was a decrease in HDL levels in all treatments, without interference of the CPA. The ALT activity increased in the treatment with the AFL and decreased in the AFO. Therefore, we conclude that C. peltophoroides extracts, although not hepatoprotective, it has presented as antimutagenic.
Downloads
References
Arif T, Mandal TK, Kumar N, Bhosale JD, Hole A, Sharma GL, Padhi MM, Lavekar GS, Dabur R (2009) In vitro and in vivo antimicrobial activities of seeds of Caesalpinia bonduc (Lin.) Roxb. J Ethnopharmacol 123(1):177-180. https://doi.org/10.1016/j.jep.2009.02.040
Bastos VGA (2011) Avaliação da atividade farmacológica de Caesalpinia echinata Lam. (flores). 100p. Dissertação (Mestrado – área de concentração em Ciências Farmacêuticas), Universidade Federal de Pernambuco, Recife, Brazil.
Bueno FG, Panizzon GP, Mello EVSL, Lechtenberg M, Petereit F, Mello JCP, Hensel A (2014) Hydrolyzable tannins from hydroalcoholic extract from Poincianella pluviosa stem bark and its wound-healing properties: phytochemical investigations and influence on in vitro cell physiology of human keratinocytes and dermal fibroblasts. Fitoterapia 99:252-260. https://doi.org/10.1016/j.fitote.2014.10.007
Deharo E, Bourdy G, Quenevo C, Muñoz V, Ruiz G, Sauvain M (2001) A search for natural bioactive compounds in Bolivia through a multidisciplinary approach. Part V. Evaluation of the antimalarial activity of plants used by the Tacana Indians. J Ethnopharmacol 77(1):91-98. doi:10.1016/s0378-8741(01)00270-7
Delmanto RD, de Lima PL, Sugui MM, da Eira AF, Salvadori DM, Speit G, Ribeiro LR (2001) Antimutagenic effect of Agaricus blazei Murrill mushroom on the genotoxicity induced by cyclophosphamide. Mutat Res 496(1-2):15-21. https://doi.org/10.1016/s1383-5718(01)00228-5
Duell PB, Oram JF, Bierman EL (1991) Nonenzymatic glycosylation of HDL and impaired HDL-receptor-mediated cholesterol efflux. Diabetes 40(3):377–384. https://doi.org/10.2337/diab.40.3.377
Ekor M (2014) The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol 4:177. https://doi.org/10.3389/fphar.2013.00177
França ISX, Souza JA, Baptista RS, Britto VRS (2008) Medicina popular: benefícios e malefícios das plantas medicinais. Rev Bras de Enferm 61(2):201-208. https://doi.org/10.1590/S0034-71672008000200009
Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical chemistry, 18(6):499–502.
Gobbo-Neto L, Lopes NT (2007) Plantas medicinais: fatores de influência no conteúdo de metabólitos secundários. Quim Nova 30(2):374-381.
Gomes-Carneiro MR, Dias DM, De-Oliveira AC, Paumgartten FJ (2005) Evaluation of mutagenic and antimutagenic activities of alpha-bisabolol in the Salmonella/microsome assay. Mutat Res 585(1-2):105–112. https://doi.org/10.1016/j.mrgentox.2005.04.007
Gouveia-Matos JAM (1999) Mudança nas cores dos extratos de flores roxas e do repolho roxo. Quím Nova Escola 10:06-10.
Harborne JB (1994) Phenolics. In: Mann J, Davidson RS, Hobbs JB (ed). Natural products: Their chemistry and biological significance. 1.ed. London: Longman. 361-388.
Hayashi M, Tice RR, MacGregor JT, Anderson D, Blakey DH, Kirsh-Volders M, Oleson Jr FB, Pacchierotti F, Romagna F, Shimada H (1994) In vivo rodent erythrocyte micronucleus assay. Mutat Res 312(3):293–304. https://doi.org/10.1016/0165-1161(94)90039-6
Jiang RW, Ma SC, But PP, Mak TC (2001) New antiviral cassane furanoditerpenes from Caesalpinia minax. J Nat Prod 64(10):1266–1272. https://doi.org/10.1021/np010174+
Kayano ACAV, Lopes SCP, Bueno FG, Cabral EC, Souza-Neiras WC, Yamauchi LM, Foglio MA, Eberlin MN, Mello JCP, Costa FTM (2011) In vitro and in vivo assessment of the anti-malarial activity of Caesalpinia pluviosa. Malar J 10:112-122. https://doi.org/10.1186/1475-2875-10-112
Kb P, Harish B, Ch M, Padmavathi T. (2012) Evaluation of nephroprotective activity of the methanolic extract of leaves of Bauhinia variegata Linn, (Family-Caesalpiniaeae). J Pharma Sci Tech 2(1):16-19.
Liu AL, Shu SH, Qin HL, Lee SM, Wang YT, Du GH (2009) In vitro anti-influenza viral activities of constituents from Caesalpinia sappan. Planta Med 75(4):337–339. https://doi.org/10.1055/s-0028-1112208
Madingou NOK, Traore A, Souza A, Mounanga MMB, Samseny RRA, Ouedraogo S, Traore AS (2016) Preliminary studies of acute and sub-chronic toxicity of the aqueous extract of Guibourtia tessmannii (Harms) J. Leonard stem barks (Caesalpiniaceae) in mice and rats. Asian Pac J Trop Biomed 6(6):506-510. http://dx.doi.org/10.1016/j.apjtb.2016.04.001
Mandal S, Hazra B, Sarkar R, Biswas S, Mandal N (2011) Assessment of the antioxidant and reactive oxygen species scavenging activity of methanolic extract of Caesalpinia crista leaf. Evid Based Complement Alternat Med eCAM:173768. https://doi.org/10.1093/ecam/nep072
Manosroi A, Akazawa H, Kitdamrongtham W, Akihisa T, Manosroi W, Manosroi J (2015) Potent antiproliferative effect on liver cancer of medicinal plants selected from the Thai/Lanna medicinal plant recipe database "MANOSROI III". Evid Based Complement Alternat Med eCAM:397181. https://doi.org/10.1155/2015/397181
Nguyen HX, Nguyen NT, Dang PH, Thi Ho P, Nguyen M, Van Can M, Dibwe DF, Ueda JY, Awale S (2016) Cassane diterpenes from the seed kernels of Caesalpinia sappan. Phytochemistry 122:286–293. https://doi.org/10.1016/j.phytochem.2015.12.018
OECD (1997) Guideline for the testing of chemicals: No. 471, Bacterial reverse mutation test; No. 473, In vitro mammalian chromosome aberration test; No. 474, Mammalian erythrocyte micronucleus test; No. 476, In vitro mammalian cell gene mutation test. Organization for Economic Cooperation and Development, Paris. 10p.
Osweiler GD (1998) Toxicologia Veterinária. 1.ed. Porto Alegre: Artes Médicas. 526p.
Pasa M (2011) Local knowledge and folk medicine: ethnobotany in Cuiabá, Mato Grosso, Brazil. Bol Mus Para Emílio Goeldi Cienc Hum 6(1):179-196. http://dx.doi.org/10.1590/S1981-81222011000100011.
Rao YK, Fang SH, Tzeng YM (2005) Anti-inflammatory activities of flavonoids isolated from Caesalpinia pulcherrima. J Ethnopharmacol 100(3):249–253. https://doi.org/10.1016/j.jep.2005.02.039
Resende FA, da Silva Almeida CP, Vilegas W, Varanda EA (2014) Differences in the hydroxylation pattern of flavonoids alter their chemoprotective effect against direct- and indirect-acting mutagens. Food Chem 155:251–255. https://doi.org/10.1016/j.foodchem.2014.01.071
Rice-Evans CA, Packer L (2003) Flavonoids in health and disease. 2.ed. London: Marcel Dekke. 467p.
Sampaio FC, Pereira M, Dias CS, Costa VC, Conde NC, Buzalaf MA (2009) In vitro antimicrobial activity of Caesalpinia ferrea Martius fruits against oral pathogens. J Ethnopharmacol, 124(2):289–294. https://doi.org/10.1016/j.jep.2009.04.034
Schumann G, Aoki R, Ferrero CA, Ehlers G, Férard G, Gella FJ, Jørgensen P J, Kanno T, Kessner A, Klauke R, Kytzia HJ, Lessinger JM, Miller WG, Nagel R, Pauwels J, Schimmel H, Siekmann L, Weidemann G, Yoshida K, Ceriotti F (2006) IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. Clin Chem Lab Med 44(9):1146–1155. https://doi.org/10.1515/CCLM.2006.212
Simões CMO, Schenkel EP, Gosmann G, Mello JCP, Mentz LA, Petrovick PR (2007) Farmacognosia: da planta ao medicamento. 6.ed. Porto Alegre: Editora da UFRGS. 1102p.
Tokarnia CH, Döbereiner J, Peixoto PV (2000) Plantas Tóxicas do Brasil. 1.ed. Rio de Janeiro: Editora Helianthus. 320p.
Vieira S (1997) Estatística experimental. 1.ed. São Paulo: Atlas, 179p.
Waters MD, Stack HF, Jackson MA, Brockman HE, De Flora S (1996) Activity profiles of antimutagens: in vitro and in vivo data. Mutat Res 350(1):109–129. https://doi.org/10.1016/0027-5107(95)00097-6
Yodsaoue O, Cheenpracha S, Karalai C, Ponglimanont C, Chantrapromma S, Fun HK, Kanjana-Opas A (2008) Phanginin A-K, diterpenoids from the seeds of Caesalpinia sappan Linn. Phytochemistry 69(5):1242–1249. https://doi.org/10.1016/j.phytochem.2007.11.013
Young DS (2001) Effects of Disease on Clinical Lab Tests. 4.ed. Washington DC: AACC Press. 1850p.
Zanin JL, de Carvalho BA, Martineli PS, dos Santos MH, Lago JH, Sartorelli P, Viegas Jr C, Soares MG (2012) The genus Caesalpinia L. (Caesalpiniaceae): phytochemical and pharmacological characteristics. Molecules 17(7):7887–7902. https://doi.org/10.3390/molecules17077887
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
