Chemical composition and antibacterial activity of essential oils
DOI:
https://doi.org/10.70151/szwxdm46Keywords:
Lamiaceae, essential oil, bacteria, minimum inhibitory concentration, minimum bactericidal concentrationAbstract
Essential oils have been used as a natural alternative in the prevention and treatment of diseases that affect human health. The aim of this work was to determine the chemical composition and antibacterial activity of the essential oils of Lavandula angustifolia, Pogostemon cablin, Rosmarinus officinalis, Thymus vulgaris, Hedyosmun brasiliense, Psidium guajava, Baccharis dracunculifolia and Schinus terebinthifolius against bacteria Staphylococcus aureus, Salmonella enteritidis, Escherichia coli and Pseudomonas aeruginosa. The essential oils were analyzed by gas chromatography coupled to mass spectrometry. The antibacterial activity was determined by microdilution in broth, showing minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The chemical composition of the evaluated oils was based on monoterpenes and sesquiterpenes. The essential oil of Thymus vulgaris was highlighted with MIC of 0.195 and MBC of 1.56 µl/ml against S. aureus, 0.195 and 50 µl/ml for S. enteritidis; 0.390 µl/ml and 0.780 µl/ml for E. coli and 0.780 and 12.5 µl/ml against P. aeruginosa, respectively. The variability of specific components of the essential oils is very interesting and of economic importance, due to the therapeutic and aromatizing potential of these oils. The oils of L. angustifólia, T. vulgaris, R. officinalis, B. dracunculifolia, P. guajava and S. terebinthifolius showed activity in vitro against S. aureus, E. coli, S. enteritidis and stronger for P. aeruginosa. The oil of Thymus vulgaris presented the best results when its antibacterial activities were evaluated. The evaluated oils had an inhibitory effect on the microorganisms studied and the gram-positive bacteria were more sensitive to the effects of the essential oils when compared to the gram-negative bacteria. The essential oils evaluated in this study represent an important alternative for controlling bacterial infections.
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References
Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectrometry. 4. ed. Allured Publishing Corporation, Carol Stream, IL, USA. 698 p.
Bakkali F, Averbeck F, Averbeck S, Idaomar B (2007) Biological effects of essential oils – a review. Food Chem Toxicol 46:446-475.
Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 94:223–253.
Cannon JB, Cantrell CL, Astatkie T, Zheljazkov VD (2013) Modification of yield and composition of essential oils by distillation time. Ind Crops Prod 41:214-220.
Clinical and Laboratory StandardS Institute (CLSI) (2003) Methodology for sensitivity testing to antimicrobial agents by dilution for aerobic growth bacteria: Approved standard – M07-A6. 23:1–81.
Fabiane K, Ferronatto R, Santos A, Onofre S (2008) Physicochemical characteristics of the essential oils of Baccharis dracunculifolia and Baccharis uncinella D.C. (Asteraceae). Rev Bras Farmacogn 18(2):197-203.
Farmacopéia Brasileira (2017) 5 ed. Brasília: Agência Nacional de Vigilância Sanitária. 1006p.
Ferronato R, Marchesan ED, Pezenti E, Bednarski F, Onofre SB (2007) Atividade antimicrobiana de óleos essenciais produzidos por Baccharis dracunculifolia D.C. e Baccharis uncinella D.C. (Asteraceae). Rev Bras Farmacogn 17:224-230.
Fonseca MCM, Lehner MS, Gonçalves MG, Paula Júnior TJ, Silva AF, Bonfim FPG, Prado AL (2015) Potential of essential oils from medicinal plants to control plant pathogens. Rev. Bras. Plantas Med.17:45-50.
Holetz FB, Pessini GL, Sanches NR, Cortez DAG, Nakamura CV, Dias Filho BP (2002) Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz 97:1027-31.
International Organization for Standardization ISO 3515 (2002) Óleo de lavanda (Lavandula angustifolia Mill.) Organization for Standardization.
International Organization for Standardization ISO 3757 (2002) Óleo de patchouli (Pogostemon cablin (Blanco) Benth.). Organization for Standardization.
Jakiemiu EA, Scheer AD, Oliveira JS, Côcco LC, Yamamoto CI, Deschamps C (2010) Study of composition and yield of Thymus vulgaris L. oil essential. Semina 31(3):683–688.
Kalemba D, Kunicka A (2003) Antibacterial and antifungal properties of essential oils. Curr Med Chem 10:813-29.
Kariminik A, Baseri-Salehi M, Kheirkhah B (2017) Pseudomonas aeruginosa quorum sensing modulates immune responses: An updated review article. Immunol Lett 190:1-6.
Kirchner K, Wisniewski Jr A, Cruz AB, Biavatti MW, Netz DJA (2010) Chemical composition and antimicrobial activity of Hedyosmum brasiliense Miq., Chloranthaceae, essential oil. Rev Bras Farmacogn 20:692-9.
Lahmar A, Bedoui A, Mokdad-Bzeouich I, Dhaouifi Z, Kalboussi Z, Cheraif I, Ghedira K, Chekir-Ghedira L (2017) Reversal of resistance in bacteria underlies synergistic effect of essential oils with conventional antibiotics. Microb Pathog 106:50–59.
Loureiro RJ, Roque F, Rodrigues AT, Herdeiro MT, Ramalheira E (2016) The use of antibiotics and bacterial resistance: brief notes on their evolution. Rev Port Sau Pub 34(1):77–84.
Modolo LV, Foglio MA (2019) Brazilian medicinal plants. CRC Press/Taylor & Francis Group, BocaRaton, Florida, USA. 358 p. https://doi.org/10.1201/b22296
Moussa SH, Tayel AA, Al-Hassan AA, Farouk A (2013) Tetrazolium/formazan test as an efficient method to determine fungal chitosan antimicrobial activity. J Mycol 2013:7. https://doi.org/10.1155/2013/753692
Moyna P, Dellacassa E, Serafini L, Rosatto M, Deagostini F (2007) Avaliaçao quimica mensal de tres ejemplares de Schinus terebinthifolius. Rev Bras Biocienc 52:1011-1013.
National Institute of Standards and Technology (2010) National Institute of Standards and Technology (NIST). Available at: http://webbook.nist.gov/chemistry/name–ser.html. Accessed on: 15 Dec 2017.
Nikoli´c M, Glamoˇclijaa J, Ferreira ICFR, Calhelha RC, Fernandes A, Markovi´c T, Markovi´c D, Giweli A, Sokovi´c M (2014) Chemical composition, antimicrobial, antioxidant and antitumoractivity of Thymus serpyllum L., Thymus algeriensis Boiss. and Thymus vulgaris L. essential oils. Ind Crops Prod 52:183–190.
Nguefack J, Budde BB, Jakobsen M (2004) Five essential oils from aromatic plants of Cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of Listeria innocua examined by flow cytometry. Lett Appl Microbiol 39:395-400. https://doi.org/10.1111/j.1472-765X.2004.01587.
Oliveira S, Nishi L, Mantovani D, Pisano Mateus G, Santos T, Baptista A, Gomes R, Bergamasco R (2019) Extratos de plantas brasileiras no controle da bactéria Staphylococcus aureus causadora da mastite contagiosa em bovinos leiteiros. Rev Tecnol 27:48-58.
Packer JF, Luz MMS (2007) Evaluation and research method for natural products inhibitory activity, Rev Bras Farmacogn 17:102-107.
Silva LL, Heldwein CG, Reetz LGB, Hörner R, Mallmann CA, Heinzmann BM (2010) Composição química, atividade antibacteriana in vitro e toxicidade em Artemia salina do óleo essencial das inflorescências de Ocimum gratissimum L., Lamiaceae. Rev Bras Farmacogn 20(5):700-705.
Silva EAJD, Silva VPD, Alves CCF, Alves JM, Souchie EL, Barbosa LCDA (2016) Harvest time on the content and chemical composition of essential oil from leaves of guava. Cienc Rural 46(10):1771-1776.
Souza TS, Ferreira MFS, Menini L, de Lima Souza JRC, Parreira LA, Cecon PR, Ferreira A. (2017) Essential oil of Psidium guajava: Influence of genotypes and environment Sci Hortic 216:38-44.
Wang L, Wu Y, Huang T, Shi K, Wu Z (2017) Chemical compositions, antioxidant and antimicrobial activities of essential oils of Psidium guajava L. leaves from different geographic regions in China. Chem Biodivers 14(9):114-170. https://doi.org/10.1002/cbdv.201700114.
Xiang H, Zhang L, Yang Z, Chen F, Zheng X, Liu X (2017) Chemical compositions, antioxidative, antimicrobial, anti-inflammatory and antitumor activities of Curcuma aromatica Salisb. essential oils. Ind Crops Prod 108:6-16. https://doi.org/10.1016/j.indcrop.2017.05.058
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