Avaliação da atividade antibiótica do extrato de lúpulo (Humulus lupulus) sobre diferentes espécies de Candida.
DOI:
https://doi.org/10.70151/t3pna712Keywords:
Candida, Hops, Herbal therapyAbstract
Hop (Humulus lupulus) is a plant from the Cannabinaceae family, which inflorescences are widely used to preserve beers and are responsible for the characteristic flavor and smell of the beverage. It is also used as an herbal remedy to treat anxiety, insomnia, loss of weight or in the control of menstrual discomforts. Its antibiotic activity is also reported, but with controversial results. The present study evaluated the inhibitory capability of hops extract on Candida species in standardized tests. The hops extract was obtained by hidro-alcoholic extraction and the influence on the fungus growing was evaluated by experiments of minimum inhibitory concentration (MIC), as well as with sensibility on plate test and disc diffusion method. Moreover, we performed a synergism test between the hops extract and antifungal drugs such as Cetoconazol and Cotrimoxazol. In face of results, we conclude that hops cannot constrain the growing of Candida species, and besides that, it presents an antagonist effect on Cetoconazol, which is used to treat severe fungal infections. It also did not present any synergic or antagonist effect on cotrimaxazol.
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Aghamiri V, Mirghafourvand M, Mohammad-Alizadeh-Charandabi S, Nazemiyeh H (2016) The effect of Hop (Humulus lupulus L.) on early menopausal symptoms and hot flashes: A randomized placebo-controlled trial. Complement Ther Clin 23:130–135. https://doi.org/10.1016/j.ctcp.2015.05.001
Almeida LFD, Cavalcanti YW, Castro RD, Lima EO (2012) Atividade antifúngica de óleos essenciais frente a amostras clínicas de Candida albicans isoladas de pacientes HIV positivos. Rev Bras. Plantas Med 14(4):649–655. https://doi.org/10.1590/S1516-05722012000400012
Bocquet L, Sahpaz S, Rivière C (2018) An Overview of the Antimicrobial Properties of Hop. In: Mérillon JM., Riviere C. (eds) Natural Antimicrobial Agents: Sustainable Development and Biodiversity, vol 19. Springer, Cham. https://doi.org/10.1007/978-3-319-67045-4_2
Chadwick LR, Pauli GF, Farnsworth NR (2006) The pharmacognosy of Humulus lupulus L. (hops) with an emphasis on estrogenic properties. Phytomedicine. 13(1–2): 119–131 https://doi.org/10.1016/j.phymed.2004.07.006.
Costa CR (2009) Candida virulence factors of immunocompromised patients. Molecular characterization of Candida albicans resistant and susceptible to fluconazole. 2009. 77 f. Tese (Doutorado em Ciencias da Saude) - Universidade Federal de Goiás, Goiânia – GO, Brasil.
Taira DL, Chang MR (2011) Atividade enzimática e susceptibilidade antifúngica de Candida spp. isoladas de pacientes com candidemia em hospital universitário de Campo Grande-MS, 1998-2010. 2011. 60 f. Dissertação (Mestrado no Programa de Pós-Gradução em Doenças Infecciosas e Parasitárias) Universidade Federal do Mato Grosso do Sul, Campo Grande – MS, Brasil.
Demitto FO, Amaral RCR, Biasi RP e Guilhermetti E (2012) Suscetibilidade a antifúngicos in vitro de Candida spp. em pacientes do Hospital Universitário Regional de Maringá-PR. J Bras Patol Med Lab [online]. https://doi.org/10.1590/S1676-24442012000500003
Dutra FSG, Carlos L A, da Motta OV, Vianna AP, Pereira SMF (2016) Atividade antimicrobiana de extratos vegetais frente à bactérias de importância médica. Biológicas & Saúde 6(20). https://doi.org/10.25242/88686202016965
Gerhäuser C (2005a) Beer constituents as potential cancer chemopreventive agents. Eur J Cancer 41(13):1941-54. https://doi.org/10.1016/j.ejca.2005.04.012
Gerhäuser C (2005b) Broad spectrum anti-infective potential of xanthohumol from hop (Humulus lupulus L.) in comparison with activities of other hop constituents and xanthohumol metabolites. Mol Nutr Food Res 49(9):827-831. https://doi.org/10.1002/mnfr.200500091
Karkowska-Kuleta J, Rapala-Kozik M, Kozik A (2009) Fungi pathogenic to humans: molecular bases of virulence of Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. Acta Biochim Pol 56(2):211-24.
Keiler AM, Zierau O, Kretzschmar G (2013). Hop extracts and hop substances in treatment of menopausal complaints. Planta Medica 79(7):576–579. https://doi.org/10.1055/s-0032-1328330
Mardegan RC, Klein MI, Golvea MB, Rodrigues JAO, Gonçalves RB e Höfling JF (2006) Biotyping and genotypic diversity among oral Candida albicans strains from caries-free and caries-active healthy children. Braz J Microbiol 37(1):26-32 https://doi.org/10.1590/S1517-83822006000100005>
Mizobuchi S & Sato Y (1985) Antifungal Activities of Hop Bitter Resins and Related Compounds. Agr Biol Chem Tokyo 49(2):399-403. https://doi.org/10.1080/00021369.1985.10866749.
Nionelli L, Pontonio E, Gobbetti M, Rizzello CG (2018) Use of hop extract as antifungal ingredient for bread making and selection of autochthonous resistant starters for sourdough fermentation. Int J Food Microbiol 266:173-182. https://doi.org/10.1016/j.ijfoodmicro.2017.12.002.
Oliveira RDR, Maffei CML e Martinez R (2001) Infecção urinária hospitalar por leveduras do gênero Candida. Rev Assoc Med Bras 47(3):231-235. https://doi.org/10.1590/S0104-42302001000300035>
Nowack LD, Silva MLC, Guimarães LM, Cezar LP, Nogueira AL, Freire NMS (2009) Epidemiological and clinical aspects study and therapeutic actualization of vulvovaginosis by Candida ap., Trichomonas vaginalis and bacterial vaginosis by Gardnerella vaginalis. J. bras. med 77(5/6):46-50.
Royle D. (1992) Hops. By R. A. Neve. London: Chapman and Hall, (1991), pp. 266, Experimental Agriculture. 28(1):123-124. https://doi.org/10.1017/S0014479700023085.
Sá MCN, Sousa HR, Amaro CSO, Pinheiro DN, Oliveira MMM e Pinheiro MCN (2014) Isolamento de Candida no esfregaço cérvico-vaginal de mulheres não gestantes residentes em área ribeirinha do Estado do Maranhão, Brasil, 2012. Rev Pan-Amaz Saude 5(1):25-34. http://dx.doi.org/10.5123/S2176-62232014000100003.
Saugspier M, Dorn C, Czech B, Gehrig M, Heilmann J e Hellerbrand C (2012) Hop bitter acids inhibit tumorigenicity of hepatocellular carcinoma cells in vitro. Oncol Rep 28:1423-1428. https://doi.org/10.3892/or.2012.1925
Small, E. (1978). A Numerical and Nomenclatural Analysis of Morpho-Geographic Taxa of Humulus. Syst Bot 3(1):37–76. https://doi.org/10.2307/2418532
Sumiyoshi M, Kimura Y (2013) Hop (Humulus lupulus L.) extract inhibits obesity in mice fed a high-fat diet over the long term. Br J Nutr 109(1):162–172. https://doi.org/10.1017/S000711451200061X
Tabata N, Ito M, Tomoda H e Ömura S (1997). Xanthohumols, diacylglycerol acyltransferase inhibitors, from Humulus lupulus. Phytochemistry. 46(4):683–687. https://doi.org/10.1016/s0031-9422(97)00157-x.
Teuber M, Schmalreck AF (1973) Membrane leakage in Bacillus subtilis 168 induced by the hop constituents lupulone, humulone, isohumulone and humulinic acid. Archiv Mikrobiol. 94:159–171. https://doi.org/10.1007/BF00416690.
Zanoli P, Zavatti M, Rivasi M, Brusiani F, Losi G, Puia G, Avallone R e Baraldi M (2007) Evidence that the beta-acids fraction of hops reduces central GABAergic neurotransmission. J Ethnopharmacol 109(1):87–92. https://doi.org/10.1016/j.jep.2006.07.008
Zhao F, Nozawa H, Daikonnya A, Kondo K e Kitanaka S (2003) Inhibitors of nitric oxide production from hops (Humulus lupulus L.). Biol Pharm Bull 26(1):61–65. https://doi.org/10.1248/bpb.26.61.
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