Efeito do seccionamento de explantes no cultivo in vitro de babosa (Aloe vera L.)
DOI:
https://doi.org/10.70151/he6vte53Keywords:
Phytocosmetics, Tissue culture, Herbal medicines, In vitro multiplicationAbstract
Medicinal species such as Aloe vera L. present a need to optimize their in vitro culture through effective in vitro multiplication protocols, which suppress demand in the pharmaceutical and cosmetic market favoring the biological diversity of Aloe vera L. The objective of this work was to evaluate the influence of the sectioning of explants on Aloe vera L. submitted to in vitro multiplication. For this, axillary buds originating from Aloe vera L. plants were used as explants. To evaluate the influence of explant sectioning on in vitro multiplication, non-sectioned explants were cultured and sectioned in MS medium with 0.2 mg/l of ANA and 2.0 mg/l of BAP. The shoots were submitted to multiplication by means of three successive repetitions with interval of 30 days each. The experimental design was completely randomized and in a 3 x 2 factorial scheme (three subcultures and two types of explants), with 5 replicates per treatment, the experimental unit being represented by a vial containing three explants. The following characteristics were evaluated: plant height, number of shoots, number of green leaves, presence of senescent leaves and presence of roots. Sectioned explants combined with subcultures yielded the best number of shoots and number of green leaves responses, proving to be an efficient method for in vitro multiplication of A. vera.
Downloads
References
Almeida WAB, Mourão Filho, FDAA, Mendes BMJ, Rodriguez APM (2002) In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia Sci Agric 59: 35-40.
Andersen AS (1986) Environmental influences on adventitious rooting cuttings of non-woody species. In: JACHSON, M.B. (Ed.) New root formation in plants and cutting Dordrecht: Martinus Nijhoff Publishers. 223-253.
BRASIL (2012a) Ministério da Saúde. Secretaria de Ciência, Tecnologia e Insumos Estratégicos. Departamento de Assistência Farmacêutica e Insumos Estratégicos. Relação Nacional de Medicamentos Essenciais: RENAME 2012. Brasília: Ministério da Saúde.
Carvalho ACPP de, Costa AMG, Santos MRA dos (2006) Efeito do tipo de incisão sobre a dominância apical em ápices caulinares em duas cultivares de bananeira (Musa sp.). Plant Cell Cult Micropropag 2: 48-52.
Costa CT, De Almeida MR, Ruedell CM, Schwambach J, Maraschin FDS, Fett-Neto, AG (2013) When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings. Front Plant Sci 4: 133.
Cutter EG (1986) Anatomia Vegetal: células e tecidos. São Paulo: Roca. 304p.
De Fossard RA, Baker PK, Bourne RA (1977) The organ culture of nodes of four species of Eucalyptus. Acta Hortic 78: 157-165.
Fonseca AP, Silva ECD, Pereira MB, Oliveira RPD, Dornelles ALC (2013) Estabilidade fenotípica de genótipos de morangueiro submetidos a número variável de subcultivos in vitro. Ciênc Rural 43: 1345-1350.
Gomide DG (2004) Influência do número de subcultivos na multiplicação in vitro e na aclimatização de plantas micropropagadas de morangueiro. 93p. Dissertação (Mestrado em Agronomia) Faculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo, Passo Fundo, Brasil.
Molsaghi M, Moieni A, Kahrizi D (2014) Efficient protocol for rapid Aloe vera micropropagation. Pharm Biol 52: 735-739.
Moubayidin L, Di Mambro R, Sabatini S (2009) Cytokinin–auxin crosstalk Trends Plant Sci 14: 557-562.
Moura TL, Almeida WABD, Mendes B, Januzzi M, Mourão Filho FDAA (2001) Organogênse in vitro de Citrus em função de concentrações de BAP e seccionamento do explante. Rev Bras Frutic 23: 240-245.
Ni Y, Turner D, Yates KÁ, Tizard I. (2004) Isolation and characterisation of structural components of Aloe vera L. leaf pulp. Int Immunopharmacol 4: 1745–1755.
Nointon D, Vine JH, Mullins MG (1992) Effects of serial subculture in vitro on the endogenous levels of indole-3-acetic acid and abscisic acid and rootability in microcuttings of ‘Jonathan’apple. Plant Growth Regul 11: 377-383.
Oliveira T R (2017) Influência dos subcultivos e da qualidade da luz na morfogênese in vitro em Cedrela fissilis VELL. (Meliaceae). 112p. Dissertação (Mestrado em Produção Vegetal). Universidade Federal do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Brasil.
Rescarolli CLS, Zaffari GR (2009) Produção de mudas de Etlingera elatior (Jack) R.M. Sm. Através da cultura de tecidos vegetais in vitro. Rev Bras Pl Med 11: 190- 195.
Silva CG, Debiasi C, Pescador R (2007) Enraizamento in vitro e aclimatização de mudas micropropagadas de Aloe vera L. Rev Bras Pl Med 9: 29-35.
Talmadge J, Chavez J, Jacobs L, Munger C, Chinnah T, Chow JT, Yates, K (2004) Fractionation of Aloe vera L. inner gel, purification and molecular profiling of activity. Int. Immunopharmacol 4: 1757-1773.
Zaffari GR, Soliman Filho LF, Stuker H (1994) Efeito do tamanho do explante e da quebra da dominância apical, sobre a brotação das gemas laterais na produção de mudas de bananeira, in vitro. Rev Bras Frutic 16: 71-76.
Downloads
Published
Issue
Section
License
Copyright (c) 2019 Revista Brasileira de Plantas Medicinais
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
