Anatomical changes in leaves of Piper aduncum L. under different irradiances
DOI:
https://doi.org/10.70151/bznsdt20Keywords:
Monkey pepper, Ecological anatomy, Luminosity, mesophyllAbstract
The aim of the study was to evaluate the effect of different light conditions on the internal structure of Piper aduncum L. leaves. P.aduncum seedlings were grown for 4 months under different light conditions achieved with shading nets (50, 70, and 100% of luminosity) and with photoconversion nets in red (RN) and blue (BN) colors. Two fully expanded leaves located at the third node were collected from five plants per treatment for paradermal and transverse sections. In the paradermal sections, stomatal density, polar and equatorial diameters, the ratio between polar and equatorial diameters, and trichome density on the abaxial and adaxialsurfaces of the epidermis were evaluated. In the transverse sections, the thickness of the adaxial and abaxial epidermis, cuticle of the adaxial surface, palisade and spongy parenchyma, and leaf blade were assessed. The vessel elements parameters were also evaluated and Carlquist vulnerability index were measured. The results show that P. aduncum changes the internal structure of its leaf when grown under different radiation conditions. It presented greater epidermis thickness, greater stomatal density and smaller diameters of vessel elements, anatomical characteristics favorable to its growth under high irradiances.
Downloads
References
Agustí J, Blázquez, MA (2020) Plant vascular development: mechanisms and environmental regulation. Cell Mol Life Sci 77:3711-3728. https://doi.org/10.1007/s00018-020-03496-w
Al Murad M, Razi K, Jeong BR, Samy PMA, Muneer S (2021) Light emitting diodes (LEDs) as agricultural lighting: Impact and its potential on improving physiology, flowering, and secondary metabolites of crops. Sustainability 13:1985. https://doi.org/10.3390/su13041985
Arik C, Altindişli A (2021) The effects of fixation and staining methods in histological investigation on the grafted cuttings of grapevine. J Agri Facul Gaziosmanpaşa Uni 38:65-72. https://doi.org/10.13002/jafag4762
Arroyo J, Bonilla P, Marín M, Tomás G, Huamán J, Ronceros G, Raez E, Moreno L, Hamilton W (2022) Anatomical and histochemical study of the vegetative organs of Piper aduncum L. (Piperaceae). Polibotánica 54:185-202. https://doi.org/10.18387/polibotanica
Belyaeva TN, Butenkova AN (2021) Anatomy of the leaf blades of Waldsteinia ternata (Stephan) Fritsch (Rosaceae) grown under different light conditions. Acta Biol Sib 7:381–390. https://doi.org/10.3897/abs.7.e75406
Brestic M, Zivcak M, Vysoka DM, Barboricova M, Gasparovic K, Yang X, Kataria S (2023) Acclimation of photosynthetic apparatus to UV-B radiation. UV-B radiation and crop growth. Singapore: Springer Nature Singapore 2023:223-260. https://doi.org/10.1007/978-981-19-3620-3_11
Brodribb TJ, Sussmilch F, Mc Adam SAM (2020) From reproduction to production, stomata are the master regulators.Plant J 101: 756-767.https://doi.org/10.1111/tpj.14561
Brousseau VD, Wu Bo-Sen, MacPherson S, Morello V, Lefsrud M (2021) Cannabinoids and terpenes: how production of photo-protectants can be manipulated to enhance Cannabis sativa L. phytochemistry. Front Plant Sci12. https://doi.org/10.3389/fpls.2021.620021
Durofil A, Radice M, Blanco-Salas J, Ruiz-Téllez T (2021) Piper aduncum essential oil: a promising insecticide, acaricide and antiparasitic: A review. Parasite 28:1-19. https://doi.org/10.1051/parasite/2021040
Escobar-Bravo R, Ruijgrok J, Kim HK, Grosser K, Van Dam NM, Klinkhamer PGL, Leiss KA (2018) Light intensity-mediated induction of trichome-associated allelochemicals increases resistance against thrips in tomato. Plant Cell Physiol 59:2462-2475. https://doi.org/10.1093/pcp/pcy166
Frade SRP, Souza DMSC, Fernandes SB, Avelar MLM, Molinari LV, Gonçalves DS, MagalhãesTA, Brondani GE (2023) Spectral quality influence on in vitro morphophysiological responses of Eucalyptus dunnii Maiden and Eucalyptus grandis W. Hill ex Maiden × E. urophylla ST Blake. N Z J For Sci 53:1-16 https://doi.org/10.33494/nzjfs532023x218x
Gogosz AM, Boeger MRT, Negrelle RRB, Bergo C (2012) Anatomia foliar comparativa de nove espécies do gênero Piper (Piperaceae). Rodriguésia 63:405-417. https://doi.org/10.1590/S2175-78602012000200013
Gomes JADO, Mielke MS, Costa LCDB, Oliveira RAD, Silva DDC (2023) Different levels of irradiance and plant regulators modify the leaf structure and essential oil production of Lippia origanoides HBK (Verbenaceae). Afr J Plant Sci 17:1-10. https://doi.org/10.5897/AJPS2017.1611
González Moreno A, de Cózar A, Prieto P, Domínguez E, Heredia A (2022) Radiationless mechanism of UV deactivation by cuticle phenolics in plants. Nat Commun 13:1786. https://doi.org/10.1038/s41467-022-29460-9
Guimarães EF, Medeiros EVSS, Queiroz GA (2024) Piper in Flora e Funga do Brasil. Jardim Botânico do Rio de Janeiro. Available at: <https://floradobrasil.jbrj.gov.br/FB12738>. Accessed on: May 6, 2024.
Haworth M, Marino G, Materassi A, Raschi A, Scutt CP, Centritto M (2023) The functional significance of the stomatal size to density relationship: Interaction with atmospheric [CO2] and role in plant physiological behaviour. Sci Tot Environ 863:160908. https://doi.org/10.1016/j.scitotenv.2022.160908
Khan PSSV, Kozai T, Nguyen QT, Kubota C, Dhawan V (2002) Growth and net photosynthetic rates of Eucalyptus tereticornis Smith under photomixotrophic and various photoautotrophic micropropagation conditions. Plant Cell Tissue Organ Cult 71:141-146. https://doi.org/10.1023/A:1019935208418
Kraus,JP, Arduin M (1997) Manual básico de métodos em morfologia vegetal. EDUR, São Paulo, Seropédica. 198p.
Matos FS, Wolfgramm R, Gonçalves FV, Cavatte PC, Ventrella MC, Damatta FM (2009) Plenotypic plasticity in response to ligth in the coffee tree. Environ Exp Bot 67:421–427. https://doi.org/10.1016/j.envexpbot.2009.06.018
Meng X, Wang Z, He S, Shi L, Song Y, Lou X, He D (2019) LED-supplied red and blue light alters the growth, antioxidant status, and photochemical potential of in vitro-grown Gerbera jamesonii plantlets. Kho J Hort Sci Technol 37:473-489. https://doi.org/10.7235/HORT.20190048
Momayyezi M, Borsuk AM, Brodersen CR, Gilbert ME, Théroux‐Rancourt G, Kluepfel DA, McElrone AJ (2022) Desiccation of the leaf mesophyll and its implications for CO2 diffusion and light processing. Plant Cell Environ 45:1362-1381. https://doi.org/10.1111/pce.14287
Morais VP, Cabral FV, Fernandes CC, Miranda MLD (2023) Brief review on Piper aduncum L., its bioactive metabolites and its potential to develop bioproducts. Braz Arch Biol Tech 66:1-11. https://doi.org/10.1590/1678-4324-2023220314
Moriwaki T, Falcioni R, Giacomelli ME, Gibin MS, Sato F, Nanni MR, Lima SM, Andrade LHC, Baesso ML, Antunes WC (2023) Chloroplast and outside-chloroplast interference of light inside leaves. Environ Exp Bot 208:105258. https://doi.org/10.1016/j.envexpbot.2023.105258
Negin B, Hen‐Avivi S, Almekias‐Siegl E, Shachar L, Jander G, Aharoni A (2023) Tree tobacco (Nicotiana glauca) cuticular wax composition is essential for leaf retention during drought, facilitating a speedy recovery following rewatering. New Phytol 237:1574-1589. https://doi.org/10.1111/nph.18615
Nor FAMD, Syukri Y (2020) Medicinal uses, phytochemistry, and pharmacological properties of Piper aduncum L. Sains Malays 49:1829-1851. http://doi.org/10.17576/jsm-2020-4908-07
Revathi J, Manokari M, Priyadharshini S, Shekhawat MS (2020) Foliar micromorphometric adaptations of micropropagated plants of Oldenlandia herbacea (L.) Roxb.- an important medicinal herb. Curr Sci 119:398-401. https://doi.org/10.18520/cs/v119/i2/390-398
Rocha SFR, Ming LC, Chaves FCM, Scarda FM (2008) Role of light and phytochrome on Piper aduncun L. germination: An adaptive and environmental approach. J Herb Spices Med Plant 11:85-96. https://doi.org/10.1300/J044v11n03 08
SAEG (2007) Sistema para Análises Estatísticas e Genéticas.Fundação Arthur Bernardes- UFV.
Sarroupoulou V, Sperdouli I, Adamakis ID, Grigoriadou K (2023) The use of different LEDs wavelength and light intensities for in vitro proliferation of cherry rootstock: Influence on photosynthesis and photomorphogenesis. Plant Cell Tissue Organ Cult 152:317-330. https://doi.org/10.1007/s11240-022-02408-z
Silva TD, Batista DS, Fortini EA, de Castro KM, Felipe SHS, Fernandes AM, Otoni WC (2020) Blue and red light affects morphogenesis and 20-hydroxyecdisone content of in vitro Pfaffia glomerata accessions. J Photochem Photobiol 203:111761. https://doi.org/10.1016/j.jphotobiol.2019.111761
Sousa PJC, Barros CAL, Rocha JCS, Lira DS, Monteiro GM, Maia JGS (2008) Avaliação toxicológica do óleo essencial de Piper aduncum L. Rev Bras Farmacog 18:217-221. https://doi.org/10.1590/S0102-695X2008000200013
Ueda T, Murata M, Yokawa K (2021) Single wavelengths of LED light supplement promote the biosynthesis of major cyclic monoterpenes in Japanese mint. Plants 10:1420. https://doi.org/10.3390/plants10071420
Yang J, Chong P, Chen G, Xian J, Liu Y, Yue Y (2023) Shifting plant leaf anatomical strategic spectra of 286 plants in the eastern Qinghai-Tibet Plateau: Changing gears along 1050–3070 m. Ecol Indic 146:109741. https://doi.org/10.1016/j.ecolind.2022.109741
Zhang Y, Chen C, Jin Z, Yang Z, LI Y (2022) Leaf anatomy, photosynthesis, and chloroplast ultrastructure of Heptacodium miconioides seedlings reveal adaptation to light environment. Environ Exp Bot 195:104780. https://doi.org/10.1016/j.envexpbot.2022.104780
Downloads
Additional Files
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
