Angiogenic potential of “Quina do Cerrado” (Strychnos pseudoquina)

Authors

DOI:

https://doi.org/10.70151/ny5krn38

Keywords:

Strychnos pseudoquina, Angiogenesis, Chorioallantoic membrane

Abstract

Leaf and bark infusions of the Strychnos pseudoquina A.St.-Hil., Loganiaceae, plant have long been used in Brazilian traditional medicine for the treatment of various diseases and medical conditions, showing apparent antipyretic, gastric cicatrizant, and especially antimalarial effects. The literature contains abundant reports of plants that possess bioactive components with therapeutic properties. The search for natural substances that promote or inhibit angiogenesis is the focus of current research, since numerous drugs derived from plants have these effects. Thus, in this study, we investigated the angiogenic potential of S. pseudoquina, using the embryonated chicken egg chorioallantoic membrane assay for the angiogenesis tests. Three concentrations of an aqueous extract of S. pseudoquina bark were tested: 15, 30, and 60 mg/ml. The mean percentages of vascularization of CAMs treated with these three extract concentrations were 15.4%, 17.1%, and 17.2%, respectively, which were significantly higher (p<0.05) than that in the negative (8.9%) and inhibitor controls (2.2%). Therefore, it was concluded that the aqueous extract of S. pseudoquina can promote angiogenesis at the concentrations tested.

Downloads

Download data is not yet available.

Author Biographies

  • Isteuria Cristina Paula Santos, Pontifical Catholic University of Goiás

    Laboratory of Experimental and Biotechnological Research – LEB

  • Valéria Bernadete Leite Quixabeira, Pontifical Catholic University of Goiás

    Laboratory of Experimental and Biotechnological Research – LEB

  • Fatima Mrué, Pontifical Catholic University of Goiás

    Laboratory of Experimental and Biotechnological Research – LEB

  • Nikary Stéfany Paula Santos, Pontifical Catholic University of Goiás

    School of Medical and Life Sciences

  • Clever Gomes Cardoso, Federal University of Goiás

    Department of General Biology

  • Lee Chen Chen, Federal University of Goiás

    Department of General Biology, Institute of Biological Sciences

  • Abel Vieira de Melo Bisneto, Federal University of Goiás

    Department of General Biology, Institute of Biological Sciences

  • Paulo Roberto de Melo Reis, Pontifical Catholic University of Goiás

    Laboratory of Experimental and Biotechnological Research – LEB

References

Azzarello J, Ihnat MA, Kropp BP, Warnke LA, Lin HK (2007) Assessment of angiogenic properties of biomaterials using the chicken embryo chorioallantoic membrane assay. Biomed Mater. https://doi.org/10.1088/1748-6041/2/2/001

Bernardini S, Tiezzi A, Laghezza Masci V, Ovidi E (2018) Natural products for human health: an historical overview of the drug discovery approaches. Nat Prod Res 32(16):1926-1950. https://doi.org/10.1080/14786419.2017.1356838

Bhadada SV, Goyal BR, Patel MM (2011) Angiogenic targets for potential disorders. Fundam Clin Pharmacol 25(1) 29–47. https://doi.org/10.1111/j.1472-8206.2010.00814.x

Bonamin F, Moraes TM, Kushima H, Silva MA, Rozza AL, Pellizzon CH, Bauab TM, Rocha LR, Vilegas W, Hiruma-Lima CA (2011) Can a Strychnos species be used as antiulcer agent? Ulcer healing action from alkaloid fraction of Strychnos pseudoquina St. Hil. (Loganiaceae). J Ethnopharmacol 138(1):47-52. https://doi.org/10.1016/j.jep.2011.08.020

Brandão EK de S, Rapini A (2018) Flora of Bahia: Loganiaceae. SITIENTIBUS S C B 18. https://doi.org/10.13102/scb2648

Brazil. Ministry of Health (2021) Contributions to promoting the rational use of medicines. Brasilia, Brazil. https://bvsms.saude.gov.br/bvs/publicacoes/contribuicoes_promocao_uso_racional_medicamentos_v2.pdf

Carmeliet P (2000) Mecanismos de angiogênese e arteriogênese. Nat Med 6(4):389-395. https://doi.org/10.1038/74651

Chaves DA, Lemes SR, Araujo LA, Sousa MAM, Freitas GB, Lino-Junior RS, Mrue F, Melo-Reis PR (2016) Avaliação da atividade angiogênica da solução aquosa do barbatimão (Stryphnodendron adstringens). Rev Bras Plantas Med 18(2):524–30. https://doi.org/10.1590/1983-084X/15_093

Ferreira SH, Barata LES, Filho SLM, Queiroz SRR (1998) Medicamentos a partir de plantas medicinais no Brasil. 1.ed. Rio de Janeiro: Acad Bras Cienc 131p.

Firmo WCA, Menezes VJM, Passos CEC, Dias CN, Alves LPL, Dias ICL, Neto MS, Olea RSG (2011) Historical context, popular use and scientific conception. Cad Pesq 18. Available at: https://periodicoseletronicos.ufma.br/index.php/cadernosdepesquisa/article/view/746/2578 Accessed on: 18 Mar 2024

Gontijo DC, Brandão GC, Gontijo PC, de Oliveira AB, Diaz MAN, Fietto LG, Leite JPV (2017) Identification of phenolic compounds and biologically related activities from Ocotea odorifera aqueous extract leaves. Food Chem 230:618–626. https://doi.org/10.1016/j.foodchem.2017.03.087

Gontijo DC, Nunes LG, Farias LM, Duarte MGR, Carvalho AF, Fietto LG, Leite JPV (2020) Assessment of the phenolic content, mutagenicity and genotoxicity of ethanolic extracts of stem bark and leaves from Strychnos pseudoquina A.St.-Hil. Drug Chem Toxicol 43(5):539-545. https://doi.org/10.1080/01480545.2018.1515218

Harvey A (2000) Strategies for discovering drugs from previously unexplored natural products. Drug Discov Today 5(7):294-300. https://doi.org/10.1016/s1359-6446(00)01511-7

Hori Y, Hu DE, Yasui K, Smither RL, Gresham GA, Fan TP (1996) Differential effects of angiostatic steroids and dexamethasone on angiogenesis and cytokine levels in rat sponge implants. Br J Pharmacol 118(7):1584-91. https://doi.org/10.1111/j.1476-5381.1996.tb15578.x

Koehn FE, Carter GT (2005) The evolving role of natural products in drug discovery. Nat Rev Drug Discov 4(3):206-20. https://doi.org/10.1038/nrd1657

Lage PS, de Andrade PH, Lopes Ade S, Chávez Fumagalli MA, Valadares DG, Duarte MC, Pagliara Lage D, Costa LE, Martins VT, Ribeiro TG, Filho JD, Tavares CA, de Pádua RM, Leite JP, Coelho EA (2013) Strychnos pseudoquina and its purified compounds present an effective in vitro antileishmanial activity. Evid Based Complement Alternat Med 2013:304354. https://doi.org/10.1155/2013/304354

Luay MAM, Gonzaga MFR, Po SKD, Arollado EC (2018) Determination of the antiangiogenic activity of Telescopium telescopium (horn snail) extract using in ovo chorioallantoic membrane (CAM) assay. Acta Med Philipp 52(4). Available at: https://actamedicaphilippina.upm.edu.ph/index.php/acta/article/view/379. Accessed on: 22 nov 2023.

Melo-Reis P, Andrade L, Silva C, Araújo L, Pereira M, Mrue F, Chen-Chen L (2010) Angiogenic activity of Synadenium umbellatum Pax latex. Braz J Biol 70(1):189-194. https://doi.org/10.1590/S1519-69842010000100026

Mendonça RJ (2004) Caracterização biológica de uma fração angiogênica do látex natural da seringueira Hevea brasiliensis. 102p. Dissertação (Mestrado em Bioquímica), Faculdade de Medicina de Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. Available at: https://www.teses.usp.br/teses/disponiveis/17/17131/tde-10052021-115953/publico/001411279.pdf. Accessed on: 18 Mar 2024.

Mendonça RJ, Coutinho-Netto J (2009) Aspectos celulares da cicatrização. An Bras Dermatol 84(3):257-262. https://doi.org/10.1590/S0365-05962009000300007

Nakao S, Hata Y, Miura M, Noda K, Kimura YN, Kawahara S, Kita T, Hisatomi T, Nakazawa T, Jin Y, Dana MR, Kuwano M, Ono M, Ishibashi T, Hafezi-Moghadam A (2007) Dexamethasone inhibits interleukin-1 beta-induced corneal neovascularization: role of nuclear factor-kappa B-activated stromal cells in inflammatory angiogenesis. Am J Pathol 171(3):1058-65. https://doi.org/10.2353/ajpath.2007.070172

Ng MF (2010) The role of mast cells in wound healing. Int Wound J 7(1):55-61. https://doi.org/10.1111/j.1742-481X.2009.00651.x

Nicoletti M, Goulart MO, de Lima RA, Goulart AE, Delle Monache F, Marini Bettolo GB (1984) Flavonoids and alkaloids from Strychnos pseudoquina. J Nat Prod 47(6):953-7. https://doi.org/10.1021/np50036a007

Papetti M, Herman IM (2002) Mechanisms of normal and tumor-derived angiogenesis. Am J Physiol Cell Physio 282(5):C947–C970. https://doi.org/10.1152/ajpcell.00389.2001

Patrício KP, Minato ACS, Brolio AF, Lopes MA, Barros GR, Moraes V, Barbosa GC (2022) Medicinal plant use in primary health care: an integrative review. Cien Saúde Colet. https://doi.org/10.1590/1413-81232022272.46312020

Pignal M, Brandão MGL (2014) Plantas Usuais dos Brasileiros / Auguste de Saint-Hilaire. (Mourão CPB, Santiago CF), 2. ed. Belo Horizonte: Fino Traço. 17p.

Pinho MSL (2017) Angiogênese: o gatilho proliferativo. Rev. Bras. Colo-Proctol 24(4):396-406. https://sbcp.org.br/pdfs/25_4/12.pdf. Accessed on: 22 dec 2023.

Ribatti D (2008) Chick embryo chorioallantoic membrane as a useful tool to study angiogenesis. Int Rev Cell Mol Biol 270:181-224. https://doi.org/10.1016/S1937-6448(08)01405-6

Ribatti D, Ennas MG, Vacca A, Ferreli F, Nico B, Orru S, Sirigu P (2003) Tumor vascularity and tryptase-positive mast cells correlate with a poor prognosis in melanoma. Eur J Clin Invest 33(5):420-425. https://doi.org/10.1046/j.1365-2362.2003.01152.x

Ribatti D, Nico B, Vacca A, Roncali L, Burri PH, Djonov V (2001) Chorioallantoic membrane capillary bed: A useful target for studying angiogenesis and anti-angiogenesis in vivo. Anat Rec 264: 317-324. https://doi.org/10.1002/ar.10021

Ribatti D, Vacca A, Nico B, Crivellato E, Roncali L, Dammacco F (2001) The role of mast cells in tumour angiogenesis. Br J Haematol 115(3):514-521. https://doi.org/10.1046/j.1365-2141.2001.03202.x

Santos WW, Almeida PM, Alves WS, Bacelar LL, Maia Filho ALM, Oliveira MDA, Ferreira DCL, Santos Filho FS, Martins FA (2021) The healing effect of the ethanol extract of the leaves of Himatanthus obovatus (Müll Arg.) Woodson. Res Soc Dev 10(8):1-14. https://doi.org/10.33448/rsd-v10i8.17461

Sarandy MM, Novaes RD, Xavier AA, Vital CE, Leite JPV, Melo FCSA, Gonçalves RV (2017) Hydroethanolic extract of Strychnos pseudoquina accelerates skin wound healing by modulating the oxidative status and microstructural reorganization of scar tissue in experimental type I diabetes. Biomed Res Int 2017:9538351. https://doi.org/10.1155/2017/9538351

Sarandy MM, Miranda LL, Altoé LS, Novaes RD, Zanuncio VV, Gonçalves RV (2018) Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats. PLoS ONE 13(4): e0195786. https://doi.org/10.1371/journal.pone.0195786

Silva PES, Damasceno AC, Furtado CO (2021) Use of medicinal plants and herbal medicines in the Brazilian Public Health System: an integrative review. Braz J Dev https://doi.org/10.34117/bjdv7n12-402

Souza FR (2020) Relação mastócitos e angiogênese em neoplasias mamárias caninas. p.49. Dissertação (Mestrado em Ciências Veterinárias). Universidade Federal de Lavras, Lavras, Brazil. Available at: http://repositorio.ufla.br/bitstream/1/41601/1/DISSERTA%c3%87%c3%83O_Rela%c3%a7%c3%a3o%20mast%c3%b3citos%20e%20angiog%c3%aanese%20em%20neoplasias%20mam%c3%a1rias%20caninas.pdf. Accessed on: 18 Mar 2024.

Souza ND, Fonseca HM, Madalena LJA (2020) The importance of nursing professionals' knowledge about the use of herbal medicines and medicinal plants: A systematic review. Rev Multidebates 4(6):270-282. Available at: https://revista.faculdadeitop.edu.br/index.php/revista/article/view/326/282. Accessed on: 18 Mar 2024.

Yoo SY, Kwon SM (2013) Angiogenesis and its therapeutic opportunities. Mediators inflamm 2013:127170. https://doi.org/10.1155/2013/127170

Yoshida WB (2005) Angiogenesis, arteriogenesis and vasculogenesis: treatment of the future for lower limb critical ischemia?. J Vasc Bras 4(4):316-318. https://doi.org/10.1590/S1677-54492005000400002

Zimmermann M, Mendes FF, Rodrigues DF, Faleiro MR, Campos GS, Araújo EG (2018) Membrana de látex natural de Hevea brasiliensis auxilia no processo de reparação tecidual em bovinos. Arq Bras Med Vet 70(03):741-748. https://doi.org/10.1590/1678-4162-9189

Downloads

Published

2024-08-07

Issue

Vol. 26 No. 1 (2024)

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Angiogenic potential of “Quina do Cerrado” (Strychnos pseudoquina). (2024). Revista Brasileira De Plantas Medicinais Brazilian Journal of Medicinal Plants, 26(1), 11-19. https://doi.org/10.70151/ny5krn38