Antimicrobial activity and toxicity of Abarema cochliacarpos against oral human microorganisms and human cells

João Paulo Cristovam Leite dos Santos; Allana Millena Alves da Silva; Gisele Nayara Bezerra da Silva; Elisangela Ramos Castanha; Vladimir da Mota Silveira Filho; Patrícia Lins Azevedo do Nascimento; Rosângela Estevão Alves Falcão

doi:10.70151/w6xfme48

Authors

João Paulo Cristovam Leite dos Santos Universidade de Pernambuco https://orcid.org/0000-0003-2578-1838
Allana Millena Alves da Silva Centro Universitário Tabosa de Almeida ASCES-UNITA https://orcid.org/0000-0002-2727-199X
Gisele Nayara Bezerra da Silva Universidade de Pernambuco https://orcid.org/0000-0001-7158-4689
Elisangela Ramos Castanha Universidade de Pernambuco https://orcid.org/0000-0002-1925-7629
Vladimir da Mota Silveira Filho Universidade de Pernambuco https://orcid.org/0000-0002-0148-1454
Patrícia Lins Azevedo do Nascimento Universidade de Pernambuco https://orcid.org/0000-0002-6335-0193
Rosângela Estevão Alves Falcão Universidade de Pernambuco https://orcid.org/0000-0002-7693-4630

DOI:

https://doi.org/10.70151/w6xfme48

Keywords:

Barbatimão, Anti-Infectious, Hemolysis, phytotherapy

Abstract

Abarema cochliacarpos popularly known as "Barbatimão" is a tree with medicinal properties, easily found in the Atlantic Forest and the Brazilian Cerrado, being little studied by scientists. This study aimed to evaluate the antimicrobial activity of 4 fractions (ethanol, hexane, ethyl acetate and methanol/water) of A. cochliacarpos against three mixed bacterial cultures of the oral cavity, and their cytotoxicity on human red blood cells. A narrative review was carried out using A. cochliacarpos and Barbatimão as descriptors to identify the main compounds with biological activities in the plant. Through a narrative review, it was seen that this species has saponin alkaloids, terpenes, flavonoids, steroids and tannins being responsible for the biological activities. Antimicrobial activity was determined by measuring the halo of inhibition of microbial growth, using ethanolic fraction at concentrations of 100, 50, and 25 mg/ml, and other fractions at concentrations of 25 and 10 mg/ml. The ethanol extract showed the best performance with halos of 10.60 mm at its lowest concentration, while ethyl acetate showed the worst performance, not forming halos in the 3 concentrations tested. The minimum inhibitory concentration (MIC) was 22 mg/ml, this concentration was able to inhibit microbial growth in all samples against the 3 inoculums. In this study, Barbatimão extracts did not present toxicity in hemolytic activity. In view of this research, it was possible to observe that Barbatimão showed antimicrobial activity and no toxicity, arousing the interest of new in vivo studies.

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Antimicrobial activity and toxicity of Abarema cochliacarpos against oral human microorganisms and human cells

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